C1细胞肝、脾原位接种及肠系膜静脉注射造模的比较  

Comparison of liver cancer models produced both by implantation in liver and spleen and mesenteric vein delivery with hepatocellular carcinoma cells

作　　者：阮思蓓[1] 李世宁[1] 倪江涛[1] 柴莉[1] 唐红[1] 刘俊[1] 张元[1] 唐明希[1] 

机构地区：[1]四川省泸州医学院病理学教研室,泸州646000

出　　处：《临床与实验病理学杂志》2013年第8期892-895,共4页Chinese Journal of Clinical and Experimental Pathology

基　　金：四川省教育厅重点项目(08ZA090);四川省科技厅应用基础项目(2010JY0130)

摘　　要：目的比较大鼠肝癌细胞系C1肝、脾原位接种及肠系膜静脉注射造模致肿瘤发生及侵袭转移的能力,筛选理想的C1肝细胞癌(hepatocellular carcinoma,HCC)发生及肺转移动物模型。方法 24只雌性BALB/cA裸鼠随机分为肝、脾原位接种及肠系膜静脉注射3组(8只/组)。观察裸鼠存活率,裸鼠肝、脾成瘤率及肿瘤病理学特征和远处脏器癌转移与强度。结果C1细胞肝原位接种组肝成瘤率100%,肿瘤呈结节状膨胀性生长,肝组织和腹壁有粘连及侵袭,肺转移率63%,镜下见肺门淋巴结转移率25%;C1细胞脾原位接种组脾成瘤率100%,肿瘤呈结节状膨胀性生长,脾组织和腹壁有粘连及侵袭,未见肝、肺转移。C1细胞肠系膜静脉注射组镜下仅见1只裸鼠有肝转移。结论以上3种不同方式构建的C1模型中,肝、脾原位接种模型均易成瘤,其中肝接种模型更适合作为HCC发生及肺转移机制和药物干预研究的动物模型。Purpose To establish liver cancer models in nude mice by injection of rat hepatocellular carcinoma cells （HCC） CI via different routes, and to study the carcinogenic and metastatic phenotypes in these liver cancer models. Methods HCC C1 ceils were injected into the liver, spleen lobe, or mesenteric vein of female BALB/cA nude mice （8 mice/group）. After the injections, the survival rate, tumor formation rate, carcinogenic features, and metastasis were comparatively studied. Results Mice that were directly injected with C1 HCC via the liver all developed liver cancer （100%）, and exhibited an expansive growing pattern, with surrounding invasion, lung metastasis （63%） and microscopically hilar lymph nodes metastasis （25%）. Mice that were injected with C1 HCC via the spleen all developed hepatocellular cancer in spleen （100%）, with surrounding invasion, but without any liver or lung metastasis. In the mice that were injected with HCC C1 via the mesenteric vein, only 1 animal developed microscopically liver metastasis. Conclu- sion The direct liver delivery of HCC C1 in female BALB/cA nude mice is a realizable method to establish a liver cancer model, and this model may serve as a valuable tool for the studies of the underlying mechanism for both carcinogenesis and metastasis, as well as therapeutic screening for liver cancer.

关 键 词：肝肿瘤 肝细胞癌 C1肝癌细胞系 癌发生 转移 裸鼠 动物模型 

分 类 号：R735.7[医药卫生—肿瘤] R-33[医药卫生—临床医学]