非酒精性脂肪性肝病大鼠血清白介素18、白介素10及其比值的变化和意义  

Change of plasma interleukin-18,interleukin-10 and interleukin-18/interleukin-10 ratio in rats with non-alcoholic fatty liver disease

作　　者：秦青[1] 周冬生[1] 梁志清[1] 张茂华[1] 李胜联[2] 

机构地区：[1]桂林医学院附属医院传染病学教研室,桂林541001 [2]桂林医学院附属医院预防医学教研室,桂林541001

出　　处：《安徽医科大学学报》2013年第9期1041-1043,共3页Acta Universitatis Medicinalis Anhui

基　　金：广西科技厅自然科学基金(编号:桂科自0991263);桂林市科学研究与技术开发计划项目(编号:20090539);广西卫生厅自筹经费科研课题项目(编号:Z2009036)

摘　　要：目的观察非酒精性脂肪性肝病(NAFLD)大鼠血清白介素(IL)-18、IL-10及其比值变化,探讨其在NAFLD发生中的意义。方法采用ELISA法检测20例正常大鼠和20例NAFLD模型大鼠血清IL-18、IL-10的水平并计算出IL-18/IL-10比值。结果 NAFLD模型组大鼠肝细胞脂肪变性、炎症较明显;血清IL-18水平、IL-18/IL-10比值明显高于正常组,而IL-10水平低于正常组(P<0.05),且IL-18水平和炎症程度相关(r=0.71,P<0.05),IL-10水平、IL-18/IL-10比值与炎症程度和脂肪变程度相关(r=-0.64,P<0.05;r=-0.46,P<0.05;r=0.70,P<0.05;r=0.61,P<0.05)。结论 NAFLD模型组大鼠IL-18水平与炎症程度呈正相关;IL-10水平与大鼠肝脏脂肪变性和炎症程度呈负相关,IL-18/IL-10比值与大鼠肝脏脂肪变性和炎症程度呈正相关,提示IL-18/IL-10比值可能对预测NAFLD的发生有一定意义。Objective To investigate the change of interleukin-18（IL-18）,interleukin-10（IL-10） and IL-18/IL-10 ratio in rats with non-alcoholic fatty liver disease（NAFLD）,and inquire their roles in the development of NAFLD.Methods The serum levels of IL-18 and IL-10 in rats with NAFLD were detected by enzyme linked immunosorbent assay（ELISA） then calculating the IL-18/IL-10 ratio.Results The change of steatosis and inflammation were notable in NAFLD;the serum levels of IL-18 and IL-18/IL-10 ratio in NAFLD were higher than that of normal group（P0.05）,while IL-10 was lower than those in normal group（P0.05）;IL-18 in NAFLD was correlated with the development of inflammation（r=0.71,P0.05）,IL-10 and IL-18/IL-10 were correlated with the development of inflammation and steatosis（r=-0.64,P0.05;r=-0.46,P0.05;r=0.70,P0.05;r=0.61,P0.05）.Conclusion IL-18 is positively correlated with the development of inflammation in rats with NAFLD;IL-10 is negatively correlated with the development of inflammation and steatosis in NAFLD,while IL-18/IL-10 ratio is positively correlated.IL-18/IL-10 plays an important role in the development of NAFLD.

关 键 词：非酒精性脂肪肝 IL-18 IL-10 IL-18 IL-10 

分 类 号：R575.5[医药卫生—消化系统]