非小细胞肺癌患者血浆TIMP-3、p16基因甲基化的检测及其临床意义  

Detection and significance of promoter hypermethylation of TIMP-3gene and p16 gene in plasma from non-small cell lung cancer patients

作　　者：李超[1] 宋超[1] 胡海亮[2] 于在诚[3] 金永堂[4] 薛绍礼[1] 

机构地区：[1]安徽医科大学医学生物工程教研室,合肥23003 [2]安徽医科大学第一附属医院输血科,合肥230022 [3]安徽医科大学第一附属医院胸普外科,合肥230022 [4]浙江大学医学院环境医学系,杭州310007

出　　处：《安徽医科大学学报》2013年第9期1088-1091,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:30471427);安徽省重点科研项目(编号:07021017)

摘　　要：目的检测非小细胞肺癌(NSCLC)患者癌组织与血浆中组织金属蛋白酶抑制因子-3(TIMP-3)、p16基因甲基化状态,研究血浆中两种基因的检测及联合检测在NSCLC筛查和鉴别诊断中的意义。方法采用甲基化特异性聚合酶链反应(MSP)技术,检测110例NSCLC患者癌组织、癌旁组织和外周血血浆TIMP-3、p16基因的甲基化状态,并对110例正常对照组血浆样品进行同样检测,比较各组检测结果。结果患者癌组织中TIMP-3、p16、联合检测的基因甲基化率分别高于癌旁组织(P<0.01);外周血血浆中TIMP-3、p16、联合检测基因甲基化率分别高于正常对照组血浆(P<0.01);血浆中TIMP-3、p16和联合检测的基因甲基化检出率与NSCLC临床分期、临床分类以及病理类型的对比差异均无统计学意义。结论对患者外周血浆TIMP-3、p16基因甲基化的联合检测可提高肺癌的检出率,为肺癌的筛查和鉴别诊断提供有价值的参考信息。Objective To detect the methylation of the tissue inhibitor of metalloproteinases-3（TIMP-3） gene and p16 gene in the plasma and tissues of non-small cell lung cancer（NSCLC） patients and to evaluate its clinical significance in screening and differential diagnosis of NSCLC.Methods A methylation-specific polymerase chain reaction（MSP） was performed for detecting the methylation of the TIMP-3 and p16 in the lung cancer tissues,paraneoplastic tissues,and plasma from 110 NSCLC patients.The determination was also conducted on 110 normal blood samples.Results The total frequency of the TIMP-3 and p16 joint detection methylation in lung cancer tissues was significantly higher than those in the paraneoplastic tissues（P0.01）.The detection rate of hypermethylation for TIMP-3,p16 and joint detection in the plasma of the 110 NSCLC cases was also higher than those in the plasma of the control group（P0.01）.The single and joint detection rate of hypermethylation in the plasma did not significantly correlate with the clinical classification,the pathologic types,and clinical staging of the NSCLC.Conclusion Joint detection for hypermethylation of TIMP-3 and p16 genes can improve the positive rate,and provide valuable information for screening and differential diagnosis of the NSCLC.

关 键 词：TIMP-3 P16 非小细胞肺癌 甲基化 甲基化特异性聚合酶链反应 

分 类 号：R734.2[医药卫生—肿瘤]