大鼠舌癌变过程中端粒酶活性变化的研究  

Telomerase activity in rat tongue carcinogenesis

作　　者：刘兴坤 何荣根[2] 周曾同[2] 陈万涛[2] 周晓健[2] 

机构地区：[1]江苏苏州市口腔医院口腔颌面外科,215005 [2]上海第二医科大学口腔医学院口腔颌面外科,200011

出　　处：《中华口腔医学杂志》2000年第6期426-429,共4页Chinese Journal of Stomatology

基　　金：国家自然科学基金资助项目!(3 9670 787)

摘　　要：目的　研究大鼠舌癌及癌前病变组织中的端粒酶活性 ,探讨其在口腔癌诊断与防治方面的应用前景。方法　采用PCR TRAP银染与ELISA技术分别对 6 9例 4NQO诱发大鼠舌癌变过程中端粒酶活性变化进行定性和定量检测。结果　两种方法检测端粒酶活性有很好的一致性。随口腔癌变进展 ,端粒酶阳性率递增 ,正常粘膜、单纯性增生、轻中度异常增生、重度异常增生、原位癌、鳞癌的阳性率分别为 0 0 %、12 5 %、2 5 0 %、6 6 7%、72 7%和 93 8%。结论　端粒酶激活是口腔癌形成的重要环节 ,检测癌前病变组织中端粒酶活性可预测癌变潜能。Objective To detect the telomerase activity in rat tongue cancer and precancerous lesions and to investigate the possibility of telomerase as oral cancer biological marker Methods A total of 69 rat tongue carcinogenesis specimens induced by 4 nitroquinoline 1 oxide (4NQO) were examined for telomerase activity by PCR telomeric repeat amplification protocol (TRAP) silver staining and ELISA Results Sequential increase in telomerase activity in tongue carcinogenesis was found The positive rate of telomerase in normal, HP, mmDP, sDP, ISC and SCC was 0 0%, 12 5%, 25 0%, 66 7%, 72 7% and 93 8% Conclusions Telomerase may be a tumor specific gene marker, and may play an important role in early diagnosis and treatment of oral cancer

关 键 词：大鼠 舌癌 端粒酶 癌前病变 活性 

分 类 号：R739.860.2[医药卫生—肿瘤]