逆萎康对慢性萎缩性胃炎大鼠胃黏膜保护作用的研究  

The Research of Niweikang in protecting gastric mucosa of rats with chronic atrophic gastritis

作　　者：周广玺[1] 魏文超[1] 张红珠[1] 张翠萍[1] 孙向红[1] 王辉明[1] 梁坤[1] 

机构地区：[1]青岛大学医学院附属医院消化科,山东青岛266003

出　　处：《中国中西医结合消化杂志》2013年第8期407-410,共4页Chinese Journal of Integrated Traditional and Western Medicine on Digestion

摘　　要：[目的]探讨逆萎康对慢性萎缩性胃炎(chronic atrophic gastritis,CAG)大鼠胃黏膜的保护作用。[方法]将60只健康雄性Wistar大鼠随机分为空白对照组、模型组、逆萎康高、中、低剂量干预组及替普瑞酮干预组,每组10只。模型组给予幽门螺杆菌(Hp)悬液和乙醇-水杨酸钠溶液造模14周,逆萎康干预组、替普瑞酮干预组在造模的同时分别给予逆萎康、替普瑞酮干预治疗14周,空白对照组仅给予等量0.9%氯化钠灌胃14周。观察各组大鼠胃黏膜大体形态和组织学表现,检测各组大鼠胃黏膜中氨基己糖的含量,并应用Elisa法测定各组大鼠一氧化氮(NO)、超氧化物歧化酶(SOD)及Bcl-2的含量。[结果]逆萎康干预组、替普瑞酮干预组中NO、SOD、氨基己糖含量均较模型组大鼠高(P<0.05);Bcl-2均较模型组低(P<0.05)。而逆萎康干预组NO、SOD、Bcl-2及氨基己糖含量与替普瑞酮干预组比较差异无统计学意义(P>0.05)。[结论]逆萎康与替普瑞酮一样对CAG大鼠的胃黏膜具有保护作用,可能是通过增加NO、SOD、氨基己糖含量、降低Bcl-2含量实现的。[Objective]To investigate Niwekang in protecting gastric mucosa of rats with chronic atrophic gastritis （CAG）. [Methods] Sixty healthy male Wistar rats were randomly assigned into normal control group （n= 10）, CAG model group （n= 10）, Niweikang intervention group （n= 30） and teprenone intervention group （n= 10）. Rats in CAG model group were administered intragastrically with Helicobacter pylori suspension and alcohol-sodium salicylate solution sequentially to construct CAG model for 14 weeks. Rats in Niweikang intervention group and teprenone intervention group were given additional Niweikang and te- prenone for 14 weeks, respectively. Rats in normal control group were treated with normal saline with the same volume as the model group for 14 weeks. Gross pathological and histopathological changes in gastric mucosa was observed,and the content of gastric hexosamine in 4 groups were detected, using the method of ELISA. The content of NO,SOD and Bcl2 in serum of 4 groups were detected [Results] The content of NO,SOD and hexosamine in Niweikang intervention group, teprenone intervention group and normal control group was higher than those in CAG model group,and the differences were statistically significant （P〈0. 05）;the content of Bcl2 in these three groups were lower than that in CAG model group, and the difference was statistically significant （P〈0. 05）. However, the differences of SOD, NO, Bcl2 and hexosamine between Niweikang intervention group and teprenone intervention group had no statistical difference （P〉0. 05）. [Conclusion]Niweikang has a similar protective effect to teprenone on gastric mucosa of rats, and the mechanisms may be related to the up regulation of NO, SOD, hexosamine and down regulation of Bcl2.

关 键 词：胃炎 慢性萎缩性 逆萎康 氨基己糖 一氧化氮 超氧化物歧化酶 BCL-2 

分 类 号：R573.3[医药卫生—消化系统]