肝癌组织中HBV感染与甲基化转移酶表达的关系  

Relationship between HBV infection and DNMT expression in liver cancer tissues

作　　者：张吉才[1] 余宗涛[1] 吕军[1] 李海平[1] 高波[1] 

机构地区：[1]湖北医药学院附属太和医院检验科,湖北十堰442000

出　　处：《临床肝胆病杂志》2013年第8期620-623,共4页Journal of Clinical Hepatology

基　　金：湖北省教育厅重大项目资助(Z20082401)

摘　　要：目的检测肝癌患者癌组织、肝癌旁组织、肝硬化相关组织中甲基化转移酶(DNMT)的表达情况,分析HBV感染与DN-MT表达之间的关系。方法根据是否存在HBV感染,将44例标本分成两组。HBV感染相关组:血液或组织中至少可检测出一种HBV感染相关的标志物;非HBV感染相关组:血液或组织中未检测出HBV感染相关标志物。应用荧光定量PCR(FQ-PCR)检测分期及分化程度不同的肝癌患者癌组织、肝癌旁组织、肝硬化组织中DNMT基因mRNA的表达水平。不同组间DNMTs表达水平的差异采用ANOVA分析;非参数检验方法为Spearman等级相关分析。结果 HBV感染相关组中癌组织的DNMT1、3A和3B mRNA表达水平明显高于非HBV感染相关组(P=0.001、0.006、0.02);HBV感染相关组中肝硬化组织的DNMT1、3A和3B mRNA表达水平也明显高于非HBV感染相关组(P=0.007、0.008、0.045);即使在癌旁组织,HBV感染相关组织中DNMT1、3A mRNA表达水平明显高于非HBV感染相关组织(P=0.01、0.04)。HBV感染与DNMTs表达相关分析结果显示:HBV感染与DNMT表达相关(P=0.009、0.006、0.03);HBV感染有关指标中HBsAg、HBeAg、HBV-DNA与DNMT1表达密切相关(P=0.008、0.032、0.006)。结论(1)HBV感染相关组中癌组织及肝硬化组织的DNMT1、3A和3B mRNA表达水平明显高于非HBV感染相关组。(2)HBV感染可能刺激DNMT的表达,尤其是DNMT1、3A和3B,进而促进部分抑癌基因甲基化。Objective To investigate the expression of DNA methyltransferases（DNMTs） in cancerous tissues,paracancerous tissues,and cirrhotic tissues among patients with liver cancer and to analyze the relationship between HBV infection and DNMT expression.Methods Forty-four samples were divided into HBV infection group and non-HBV infection group according to whether at least one marker of HBV infection could be detected in blood or tissue.FQ-PCR was used to measure the mRNA expression levels of DNMTs in the cancerous tissues,paracancerous tissues,and cirrhotic tissues with different tumor stages and differentiations.The differences in DNMT expression between groups were determined by analysis of variance;the relationship between HBV infection and DNMT expression was evaluated by Spearman rank correlation analysis.Results Compared with the non-HBV infection group,the HBV infection group had significantly higher mRNA expression levels of DNMT1,3A,and 3B in cancerous tissues（P=0.001,0.006,and 0.02） and in cirrhotic tissues（P=0.007,0.008,and 0.045） and significantly higher mRNA expression levels of DNMT1 and 3A in paracancerous tissues（P=0.01 and 0.04）.There were significant associations between HBV infection and DNMT expression（P=0.009 for DNMT1,0.006 for DNMT3A,and 0.03 for DNMT3B）;HBsAg,HBeAg,and HBV-DNA were closely related to DNMT1 expression（P=0.008,0.032,and 0.006）.Conclusion The liver cancer patients with HBV infection have significantly higher mRNA expression levels of DNMT1,3A,and 3B in cancerous and cirrhotic tissues than those without HBV infection.HBV infection may stimulate the expression of DNMTs,especially DNMT1,3A,and 3B,thus promoting methylation of some tumor-suppressor genes.

关 键 词：肝肿瘤 甲基转移酶类 肝炎病毒 乙型 基因表达 

分 类 号：R735.7[医药卫生—肿瘤]