废弃烟梗固态发酵生产假丝酵母  

Producing Candida.sp. HZ19 with Waste Tobacco Stems by Solid State Fermentation

作　　者：宋静[1] 申永防[1] 臧晓静[1] 王静[1] 吴扬帆[1] 张国帅[1] 刘畅[1] 朱大恒[1] 

机构地区：[1]郑州大学生物工程系,郑州市科学大道100号450001

出　　处：《烟草科技》2013年第8期75-78,共4页Tobacco Science & Technology

基　　金：河南省烟草专卖局科技攻关项目(HYKJ201021);郑州大学大学生创新创业训练计划资助项目"基于代谢调控的深红酵母发酵废烟梗产类胡萝卜素响应面优化研究"(2012cxsy107);河南省教育厅科学技术研究重点项目"烟草废弃物发酵联产单细胞蛋白和草炭技术研究"(13B180358)

摘　　要：为资源化利用废弃烟梗,以废弃烟梗为底物固态发酵生产假丝酵母。在单因子试验的基础上,利用Plackett-Burman设计法筛选出影响假丝酵母HZ19生物量的重要影响因子。结果表明,最适发酵条件为温度30℃、发酵时间3 d、接种量100 mL/kg、装载量10 g/250 mL、烟梗大小4.0～5.0 cm;通过Plackett-Burman试验分析,蔗糖、酵母粉、葡萄糖对假丝酵母HZ19生物量有显著影响,试验中最大生物量干质量为103.24 g/kg。To better utilize waste tobacco stems, a strain of Candida.sp. HZ19 was produced with waste tobacco stems by solid state fermentation. The key factors affecting the biomass of Candida.sp. HZ19 were further screened with Plackett-Burman design based on the monofactorial test. The results showed that the optimal fermentation conditions were 30 % for 3 days, inoculation rate 100 mL/kg, loading rate 10 g/250 mL and stem size 4-5 cm in length. Sucrose, yeast powder and glucose imparted significant effects on HZ19 biomass. Under Plackett-Burman design conditions, the maximum yield of biomass reached 103.24 g/kg （dry weight）.

关 键 词：废弃烟梗 固态发酵 生物量 Plackett—Burman设计 

分 类 号：TS49[农业科学—烟草工业]