高效液相色谱法测定人体血浆中他林洛尔的浓度  

HPLC determination of talinolol in human plasma

作　　者：颜苗[1] 张金娇 李焕德[1] 李兰芳[1] 郑媚[3] 

机构地区：[1]中南大学湘雅二医院临床药学研究室,湖南长沙410011 [2]广西梧州制药股份有限公司,广西梧州543002 [3]广西医科大学附属第一医院,广西南宁530000

出　　处：《中国医院药学杂志》2013年第16期1315-1318,共4页Chinese Journal of Hospital Pharmacy

基　　金：国家自然科学基金青年基金(编号:81202985);中央高校基本科研业务管理费中南大学青年教师助推项目(编号:2012QNZT151)

摘　　要：目的:建立一种灵敏、快速、简便测定人体血浆中他林洛尔浓度的高效液相色谱-紫外检测法,为研究他林洛尔在人体内的药动学提供有效的测定方法。方法:血浆经叔丁基甲醚萃取,以普萘洛尔为内标,采用Luna C18色谱柱(4.6 mm×250mm,5μm),乙腈-甲醇-10 mmol.L-1醋酸铵(25∶10∶65)为流动相,流速为1.0mL.min-1,UV检测波长为248 nm,柱温38℃。结果:他林洛尔浓度在8～500 ng.mL-1范围内线性良好(r2=0.999 9),最低定量限8.0ng.mL-1。平均萃取回收率是82.3%,平均方法回收率是106.1%,符合生物样品分析要求。结论:本方法简便、快速、灵敏,可用于他林洛尔药动学相关研究。OBJECTIVE To establish a rapid and sensitive HPLC-UV method for determination of talinolol in human plasma and assessment of the pharmacokinetics of talinolol tablets. METItODS The analytes in plasma were extracted by tert-butyl methyl ether as the solvent after samples had been spiked with propranolol. The analytical column was packed with a Phenome- nex C18 （4.6 mm×250 mm, 5 μm）. The mobile phase was acetonitrile-methyl alcohol-10 mmol.L- 1 ammonium acetate acid （25 ：10：65）, with a flow rate of 1.0 mL. min-1. The UV detection wavelength was 248 nm and the temperature was 38 ℃. RESULTS Excellent linear relationship was obtained from the range of 8 ng.mL-1 to 500 ng.mL-1 （r2 = 0. 999 9）. The limit of detection （LOD） was 8. 0 ng. mL-1 for talinolol. The average extraction recovery was above 82. 3%, and the methodology recovery was above 106. 1%. Talinolol was fitted demands of biological sample. CONCLUSION The method is accurate, sen- sitive and simple for the pharmacokinetiec study of talinolol.

关 键 词：高效液相色谱 他林洛尔 药动学 

分 类 号：R969.1[医药卫生—药理学]