食源性金黄色葡萄球菌9种肠毒素基因的多重PCR检测  

Multiplex PCR for the detection of the 9 enterotoxin genes in foodborne staphylococcus aureus

作　　者：卫沛楠[1] 吕国平[1] 徐保红[1] 周吉坤[1] 

机构地区：[1]石家庄市疾病预防控制中心,河北石家庄050000

出　　处：《现代预防医学》2013年第17期3269-3272,共4页Modern Preventive Medicine

摘　　要：目的建立多重PCR方法检测食源性金黄色葡萄球菌9种肠毒素基因,了解其在食源性金黄色葡萄球菌中的分布状况。方法建立多重PCR方法检测144株食源性金黄色葡萄球菌的9种肠毒素基因,对其分布状况进行研究。结果建立的多重PCR方法特异、高效,9种肠毒素基因在144株食源性金黄色葡萄球菌中的检出率由高到低依次为SEU(25.69%)、SEG(22.22%)、SEM(20.83%)、SEK(20.14%)、SEQ(18.06%)、SEH(15.97%)、SEN(10.42%)、SEJ(8.33%)、SEL(5.56%);52.78%的菌株含有该9种肠毒素基因中的至少1种,34.03%的菌株含有9种肠毒素基因中的两种或两种以上。结论该多重PCR方法特异性高,快速简便,可用于金黄色葡萄球菌肠毒素基因的分布研究;144株食源性金黄色葡萄球菌中9种肠毒素基因均有检出,其中以SEU、SEG、SEM、SEK检出率较高。OBJECTIVE To establish a multiplex PCR method for detecting 9 enterotoxin genes in foodborne staphylococcus aureus, and study the distribution of 9 enterotoxin genes in foodborne staphylococcus aureus. METHODS Two multiplex PCR methods were established to detect 9 enterotoxin genes in 144 foodborne staphylococcus aureus strains, and analyzed their distribution. RESULTS The multiplex PCR method was specific, efficient; The detection rates of 9 enterotoxin genes in 144 foodborne staphylococcus aureus strains were in descending order for SEU （25.69%）, SEG （22.22%）, SEM （20.83%） SEK （20.14%）, SEQ （18.06%）, SEH （15.97%）, SEN （10.42%）, SEJ （8.33%）, SEL （5.56%）; 52.78% of the strains contained at least one gene of these 9 enterotoxin genes, 34.03% of the strains contained two or more genes of these 9 enterotoxin genes. CONCLUSION The multiplex PCR method is highly specific, simple and convenient; It can be used for the study of the enterotoxin genes distribution in staphylococcus aureus; All 9 enterotoxin genes have been detected in 144 foodborne staphylococcus aureus strains, in which the detection rates of SEU, SEG, SEM, SEK are higher.

关 键 词：肠毒素基因 金黄色葡萄球菌 食源性致病菌 多重PCR 

分 类 号：R378.11[医药卫生—病原生物学]