大鼠下颌骨牵张成骨的蛋白质组学分析  

Analysis of rat mandibular distraction osteogenesis protein expression by proteomics technology

作　　者：隋健夫 刘冰[2] 邓天政[2] 逄键梁[2] 雷德林[3] 

机构地区：[1]第四军医大学口腔医院颌面外科,硕士生陕西710032 [2]解放军空军总医院口腔科,主治医师北京100036 [3]第四军医大学口腔医学院颌面外科,主任陕西710032

出　　处：《口腔颌面修复学杂志》2013年第1期1-5,共5页Chinese Journal of Prosthodontics

基　　金：国家自然科学青年基金(项目编号:81000423)

摘　　要：目的:探讨用蛋白质组学iTRAQ技术分析大鼠下颌骨牵张成骨过程中新生组织蛋白表达的改变。方法:6只大鼠进行单侧下颌骨牵张,速率:0.4mm/d,牵张期为10d,术后随机分为2组,分别于下颌骨牵张成骨牵张期第10d及下颌骨牵张成骨固定期第14d取材。将取材的新生骨组织标本进行蛋白质提取及蛋白质定量检测。应用iTRAQ技术对蛋白质样本进行检测,寻找及鉴定差异蛋白。结果:应用iTRAQ技术对大鼠下颌骨牵张成骨的新生骨组织成功进行了蛋白质组学分析,质谱鉴定出置信度95%的蛋白质共567种,共鉴定出差异蛋白207个,其中上调≥1.5倍的47个,下降≤0.8倍的58个。结论:筛选出多种与牵张成骨过程中新骨形成相关的差异表达蛋白质,为进一步验证与新骨形成相关的蛋白质奠定基础。Objective： To analyze new tissue protein changes at different periods of rat mandibular distraction osteogenesis by proteomics the iTRAQ technology.Methods： Six rats underwent unilateral distraction osteogenesis with a rat of 0.4 mm/d.The rats were randomly divided into 2 groups.The rats were sacrificed at consolidation time of 10 days of the distraction period and 14 days of the fixed period.After specimens protein extraction,protein concentration was determined.Find protein expression differences by proteomics the iTRAQ technology.Results： Successful application iTRAQ new bone tissue of rat mandibular distraction osteoporosis proteomics analysis of 567 kinds confidence level of 95% the proteins identified by mass spectrometry.Two hundred and seven differentially expressed proteins were indentified,which raised more than 1.5 times is forty-seven and down 0.8 times is fifty-eight.Conclusion： Screened with distraction osteogenesis new bone formation associated differentially expressed proteins and laid the foundation for further validation and new bone formation associated protein.

关 键 词：蛋白质组学 牵张成骨 iTRAQ技术 蛋白表达 

分 类 号：R783[医药卫生—口腔医学]