人CD146+牙周膜干细胞的分选及其干细胞特性的鉴定  

Isolation and identification of human CD146 positive periodontal ligament stem cells

作　　者：朱文俊[1] 谭媛元[1] 梁敏[1] 

机构地区：[1]中山大学附属口腔医院牙周科,硕士生广东510055

出　　处：《中华老年口腔医学杂志》2013年第2期74-78,共5页Chinese Journal of Geriatric Dentistry

基　　金：国家自然科学基金(编号81170970);留学归国人员科研启动基金(编号2009134);广东省科技计划(编号2011B031800259)

摘　　要：目的:通过酶消化法及流式细胞分选技术分选CD146+牙周膜干细胞(Periodontal ligament cells,PDLCs),探讨其干细胞特性。方法:采用流式细胞技术从人牙周膜细胞中分选CD146+PDLCs,并对其进行免疫组化染色(角蛋白、波形丝蛋白及Stro-1),成骨、成脂诱导分化及克隆形成实验鉴定其干细胞特性。结果:CD146+PDLCs表达波形丝蛋白及Stro-1,具有成骨及成脂分化能力,体外培养能形成细胞克隆。结论:以CD146为分选指标的流式细胞分选技术可作为牙周膜干细胞筛选的方法。Objectives： To isolate and culture CD146 positive periodontal ligament cells （CD146＋PDLCs） by using type I collagenase digestion and flow cytometric sorting, in order to verify the stem cell properties of CD146＋PDLCs. Methods： CD146＋PDLCs were isolated by flow activated cell sorter fi＇om human PDLCs. The stem cell properties of CD146＋PDLCs were tested by immunohistochemical staining, osteogenic/adipogenic induction and colony-forming assay. Results： CD146＋PDLCs, expressing mesenchymal stem cell surface marker Stro-1, were able to form calcium nodules/lipid droplets under osteogenic/adipogenic induction and were capable of forming colonies. Conclusions： CD 146＋PDLCs shared the similar properties with periodontal ligament stem cells, which providing a new method for periodontal ligament stem cell isolation.

关 键 词：牙周膜细胞 CD146 成骨分化 成脂分化 

分 类 号：R780.2[医药卫生—口腔医学]