人类组织激肽释放酶基因6在子宫颈癌组织中表达的研究  

Study on the expression of human tissue kailikrein gene 6 in cervical cancer tissues

作　　者：蒙秋[1] 吴清萍[1] 黄守国[1] 

机构地区：[1]中南大学湘雅医学院附属海口医院妇产科,570208

出　　处：《中国医师进修杂志》2013年第24期1-3,共3页Chinese Journal of Postgraduates of Medicine

基　　金：海南省自然科学基金（812171）

摘　　要：目的探讨人类组织激肽释放酶基因6（KLK6）在子宫颈癌组织中的表达及意义。方法以甘油醛.3.磷酸脱氢酶（GAPDH）作为内参基因，采用Taqman探针实时荧光定量反转录聚合酶链反应技术分别检测80例子宫颈癌（发生转移40例、未发生转移加例）及40例正常子宫颈组织中KLK6的表达量，并分析子宫颈癌临床病理特征与KLK6表达的关系。结果KLK6在正常子宫颈组织中表达量[（1106±0.40）×10^-3]低于子宫颈癌未转移组织[（4.41±1.70）×10^-3]、子宫颈癌转移组织[（32.22±6.70）×10^-3]，差异均有统计学意义（P〈0.01）。子宫颈癌转移组织中Ⅰa、Ⅰb、Ⅱa期KLK6表达量分别为（30.42.4-5.00）×10^-3、（31.644-1.30）×10^-3、（33.024-8.00）×10^-3，两两比较差异均无统计学意义（P〉0.05）；子宫颈癌未转移组织中Ⅰa、Ⅰb、Ⅱa期KLK6表达量分别为（4.124-1.10）×10^-3、（4.354-1.30）×10^-3、（4.824-1.90）×10^-3，两两比较差异均无统计学意义（P〉0.05）；但Ⅰa、Ⅰb、Ⅱa期子宫颈癌转移组织中KLK6表达量与同期子宫颈癌未转移组织比较差异均有统计学意义（P〈0.01）。结论KLK6可促进子宫颈癌细胞的增殖，并且参与子宫颈癌的转移过程。Objective To study the expression and significance of human tissue kallikrein gene 6 （KLK6） in cervical cancer tissues. Methods With glyceraldehyde 3-phosphate dehydrogenase （GAPDH） as reference,the expression of KLK6 in 80 cases of cervical cancer tissues （40 cases with metastasis and 40 cases without metastasis） and 40 cases of normal cervical tissues was determined by Taqman probe real-time quantitative reverse transcription-polymerase chain reaction, and analyzed the relationship between cervical cancer occurring and KLK6 expression with clinical data and pathological data, Resulls The expression of KLK6 in normal cervical tissues[（1.06±0.40）×10^-3] was lower than that in cervical cancer tissues without and with metastasis[（4.41±1.70）×10^-3, （32.22±6.70） ×10^-3], and there was significant difference （P〈 0.01）. The expression of KLK6 in Ⅰa, Ⅰb, Ⅱa stage of cervical cancer tissues with metastasis was （30.42±5.00）×10^-3, （31.64±1.30）×10^-3, （33.02±8.00） ×10^-3, and there was no significant difference among them （P 〉 0.05）. The expression of KLK6 in Ⅰa, Ⅰb, Ⅱa stage of cervical cancer tissues without metastasis was （4.12±1.10）×10^-3, （4.35±1.30）×10^-3, （4.82 ＋ 1.90）×10^-3, and there was no significant difference among them （P〉 0.05）. There was significant difference in the expression of KLK6 in Ⅰa, Ⅰb,Ⅱa stage between cervical cancer tissues with metastasis and cervical cancer tissues without metastasis （P〈0.01）. Conclusion KLK6 can stimulate the cervical cancer cell proliferation,and participate in theprogresses of cervical cancer metastasis.

关 键 词：宫颈肿瘤 组织激肽释放酶类 

分 类 号：R737.33[医药卫生—肿瘤]