瘢痕组织细胞内肌动蛋白的实验研究  

THE EXPERIMENTAL STUDY OF INTRACELLULAR ACTIN IN THE SCAR FIBROBLASTS

作　　者：张群[1] 崔磊[1] 钱云良[1] 

机构地区：[1]第二医科大学附属第九人民医院整形外科,上海200011

出　　处：《中国修复重建外科杂志》2000年第5期275-277,共3页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：国家自然科学基金!资助项目 (395 70 72 3)

摘　　要：目的　探讨增生性瘢痕、瘢痕疙瘩成纤维细胞中肌动蛋白 (actin)与瘢痕挛缩的关系。方法　取增生性瘢痕 10例 ,瘢痕疙瘩 5例 ,对成纤维细胞进行培养 ,并抽提细胞内蛋白成份后用 SDS- PAGE电泳 ,然后通过凝胶扫描仪测定两种组织来源的成纤维细胞内的总肌动蛋白、纤维状肌动蛋白 (F肌动蛋白 )、球形肌动蛋白 (G肌动蛋白 )的量 ,并计算 F/ G肌动蛋白的比值。结果　增生性瘢痕细胞每 10 4个细胞内含总肌动蛋白、F肌动蛋白、G肌动蛋白分别为 2 .38ng、0 .98ng、1.42 ng,F/ G肌动蛋白的比值为 0 .6 8,而瘢痕疙瘩分别为 1.6 8ng、0 .46 ng、1.2 6 ng及 0 .36。统计学分析 ,在总肌动蛋白、F肌动蛋白及 F/ G肌动蛋白的比值上两种瘢痕有非常显著性差异(P<0 .0 1) ,而 G肌动蛋白在两种瘢痕中无显著性差异 (P>0 .0 5 )。结论　瘢痕挛缩与细胞内总肌动蛋白、F肌动蛋白及 F/ G肌动蛋白的值密切相关 ,细胞内总肌动蛋白、F肌动蛋白及 F/ G肌动蛋白的比值不同可作为区分增生性瘢痕和瘢痕疙瘩的依据之一。Objective\ To study the relationship between intracellular actin and scar contracture. Methods\ Fibroblasts from 10 cases of hypertrophic scar and 5 cases of keloid were cultured in vitro . Total actin, filamentous actin(F actin), globular actin (G actin) and the ratio of F to G actin(F/G) were measured by densitometry after differential extraction and seperation by polyacrylamide gel electrophoresis in the presence of sodium sulfate. Results\ Total actin, F actin, G actin and F/G in hypertrophic scar fibroblasts were 2.38 ng/10 4 cells, 0.98 ng/10 4 cells, 1.42 ng/10 4 cells and 0.68 respectively, while in keloid fibroblasts were 1.68 ng/10 4 cells. 0.46 ng/10 4 cells, 1.26 ng/10 4 cells, and 0.36 respectively. There was significant differences between two tissues fibroblasts in the items of total actin, F actin, G actin, and F/G ( P<0.01) , while no significant difference in G actin( P>0.05) . Conclusion\ Total intracellular actin, F actin, and F/G may play an important role in the scar contracture. The hypertrophic scar and keloid can be distinguished by the contents of total intracellular actin, F actin and F/G.

关 键 词：瘢痕挛缩 成纤维细胞 肌动蛋白 实验研究 

分 类 号：R363[医药卫生—病理学]