激光散射法测定阿苯达唑脂质体粒度的研究  

Determination of Particle Size of Albendazole Liposome by Laser Scattering Method

作　　者：陈春燕[1] 赵军[2] 陈蓓[2] 高惠静[2] 王建华[2] 

机构地区：[1]新疆医科大学,乌鲁木齐830011 [2]新疆医科大学第一附属医院药学部,乌鲁木齐830054

出　　处：《中国药房》2013年第33期3131-3134,共4页China Pharmacy

基　　金：国家高技术研究发展计划(863计划)项目资助(No.2007AA021805);新疆医科大学第一附属医院药学专项基金资助(No.2012YX03)

摘　　要：目的:确定激光散射法测定阿苯达唑脂质体粒度的最佳条件。方法:结合透射电镜的测量结果,与不加分散剂样品进行对比,考察加入聚山梨酯80作为分散剂及分散体系的其他因素对激光散射法测定阿苯达唑脂质体粒度的影响。结果:激光散射法测定不加分散剂样品的平均粒度为(663.5±93.0)nm,而按照最佳分散条件即10%聚山梨酯80-阿苯达唑脂质体体积配比为4:1、0.9%氯化钠溶液稀释20倍时测定其平均粒度为(240.6±2.8)nm,同时透射电镜法测量样品的平均粒度为226.5nm(P>0.05)。结论:激光散射法测定阿苯达唑脂质体粒径时加入聚山梨酯80能改善脂质体团聚现象,在最佳分散条件下能获得与透射电镜基本一致的测定结果,同时不影响脂质体结构。该法稳定、快速、简便、重现性好,可用于阿苯达唑脂质体粒度测定。OBJECTIVE： To confirm the best condition for the determination of particle size of Albendazole （ABZ） liposome by laser scattering method. METHODS： Combined with the results by transmission electron microscopy and contrasted with the sample without dispersant, the influence of polysorbate 80 as dispersant and other factors of dispersed system on the determination of particle size of ABZ liposome by laser scattering method were investigated. RESULTS： The average particle size of samples with- out dispersant by laser scattering method was （663.5 ＋ 93.0） nm; that of samples was （240.6 ＋ 2.8） nrn under optimum dispersing condition： i.e. the volume ratio 10% polysorbate 80 to ABZ liposome was 4 ： 1, and was diluted by 0.9% sodium chloride for 20 times; that of samples was 226.5 nm by transmission electron microscopy （P〉0.05）. CONCLUSIONS： Polysorbate 80 is able to solve the agglomeration when laser scattering method is used for the determination of particle size of ABZ liposome. It＇s close to the result by transmission electron microscopy under optimum dispersing condition and doesn＇ t affect the structure of the liposome. The method is stable, rapid, simple and reproducible for the determination of particle size of ABZ liposome.

关 键 词：阿苯达唑脂质体 粒度 激光散射法 透射电镜 聚山梨酯80 

分 类 号：R927.1[医药卫生—药学]