中心体α-微管蛋白、P53蛋白及端粒酶在原发性肝癌中的表达及相关性  

Expressions of centrosome α tubulin,P53 protein and telomerase in hepatocarcinoma and their correlations

作　　者：李恒[1] 李哲[1] 乔晓俊[1] 高爱琴[2] 张楠[2] 

机构地区：[1]山东大学附属济南市中心医院保健科,济南250013 [2]山东大学附属济南市中心医院肿瘤科,济南250013

出　　处：《山东大学学报（医学版）》2013年第8期74-77,81,共5页Journal of Shandong University:Health Sciences

摘　　要：目的检测肝细胞肝癌、癌旁组织及肝硬化/慢性肝炎组织中中心体α-微管蛋白、P53蛋白及端粒酶的表达,探讨在肝细胞癌变过程中中心体异常与端粒酶的相关性。方法选取山东大学附属济南市中心医院病理科石蜡包埋组织肝细胞肝癌47例,相应的癌旁组织47例,肝硬化/慢性肝炎组织12例。采用免疫组织化学染色SP法检测中心体α-微管蛋白、P53蛋白及端粒酶逆转录酶hTERT蛋白;分析三者之间的相关性。结果中心体α-微管蛋白、P53蛋白在肝细胞肝癌中阳性表达率分别为59.57%、76.60%,明显高于癌旁组织23.40%、29.97%和肝硬化/慢性肝炎组织16.67%、33.33%,P均<0.05。肝癌组织中hTERT蛋白阳性表达率(68.09%)与癌旁组织(34.04%)、肝硬化/慢性肝炎组织(0%)比较差异均有统计学意义(P<0.05)。中心体α-微管蛋白、P53及hTERT三者间表达均呈正相关,提示三者在HCC癌变过程中可能存在协同作用。结论中心体异常参与了肝细胞癌变的过程,并有可能成为HCC新的诊断指标和治疗靶点。Objective To evaluate the expressions of centrosome ot tubulin, P53 protein and telomerase in human hepa- tocellular carcinoma （HCC）, paracancerous tissues, and cirrhosis / chronic hepatitis tissues, in order to investigate the correlations between centrosome abnormalities and telomerase in hepatocelhilar canceration. Methods The paraffin- embedded tissues of 47 cases of HCC, 47 paracancerous tissues and 12 cirrhosis / chronic hepatitis tissues prepared in the Pathological Department of Jinan Central Hospital were selected. Immunohistochemistry SP method was applied to detect the expressions of centrosome et tubulin, P53 protein and human telomerase reverse transcriptase （hTERT）. The correlations were then analyzed. Results The expressions of ~t tubulin in HCC, paracancerous and cirrhosis/chronic hepatitis tissues were 59.57%, 23.4% and 16.67% ; the expressions of P53 protein were 76.60%, 29.97% and 33.33%. The expressions were significantly higher in HCC than in paracancerous and cirrhosis/chronic hepatitis tissues （ P 〈 0.05 ）. The positive expressions of hTERT were 68.09% in HCC, 34.04% in paracancerous tissues and 0% in cirrhosis/chronic hepatitis tissues, with significant difference （P 〈 0.05 ）. Expressions of ct tubulin, P53 protein and telomerase were positively correlated. Conclusion Centrosome abnormalities may participate in the development of HCC, and may serve as a new target for diagnosis and therapy.

关 键 词：肝细胞肝癌 中心体 端粒酶 P53蛋白 

分 类 号：R735.7[医药卫生—肿瘤]