重组人粒细胞集落刺激因子对K562细胞凋亡和其细胞毒药物敏感性的影响  

Effect of human recombinant granulocyte-clony stimulating factor on apoptosis of K562 cell and its sensitivity to cytotoxic agents

作　　者：黄新智[1] 林洁红[1] 祁明芳[1] 

机构地区：[1]暨南大学医学院第一附属医院,510630

出　　处：《广东医学》2000年第10期821-822,共2页Guangdong Medical Journal

摘　　要：目的　探讨重组人粒细胞集落刺激因子 (rhuG -CSF)对K5 62细胞凋亡和其细胞毒药物敏感性的影响。方法　采用流式细胞术和MTT法分别测定细胞凋亡和细胞毒药物敏感性。结果　在低血清培养条件下 ,rhuG -CSF对K5 62细胞的凋亡有抑制作用 ,其凋亡百分率与rhuG -CSF浓度呈负线性相关 ;rhuG -CSF可增加K5 62细胞对Ara -C的敏感性 ,而降低其对DNR的敏感性 ;在Ara -C和DNR联合条件下 ,rhuG -CSF能增强两药的联合细胞毒毒性 ,克服了单用DNR条件下 ,其使DNR对K5 62细胞毒毒性作用降低的情况。结论　虽然rhuG -CSF并不降低DNR和Ara -C对K5 62细胞的联合细胞毒毒性 ,但当其和化疗药物同时使用治疗髓系白血病患者时 ,仍应慎重。Objective To investigate the effect of human recombinant granulocyte-clony stimulating factor (rhu G-CSF) on the apoptosis of K562 cell and its sensitivity to cytotoxic agents. Methods\ Cell apoptosis and sensitivity of cytotoxic agents were analyzed by flow cytometry and MTT assay respectively. Results\ At lower serum concentration, the rhu G-CSF had an inhibitory effect on the apoptosis of K562 cell in a negative dose dependent manner. rhu G-CSF enhanced the sensitivity of K562 cell to Ara-C while decreased its sensitivity to daunorubicin. rhu G-CSF enhanced the combinant cytotoxicity of Ara-C and daunorubicin to K562 cells. Conclusion\ rhu G-CSF can enhance the combined cytotoxicity of Ara-C and daunorubicin to K562 cells.

关 键 词：K562细胞系 细胞凋亡 白血病 rhuG-CSF 

分 类 号：R733.7[医药卫生—肿瘤]