ATPG is required for the accumulation and function of chloroplast ATP synthase in Arabidopsis  

作　　者：KONG MengMeng WANG FenFei YANG ZhongNan MI HuaLing 

机构地区：[1]National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences [2]College of Life and Environmental Sciences, Shanghai Normal University

出　　处：《Chinese Science Bulletin》2013年第26期3224-3232,共9页

基　　金：supported by the National Natural Science Foundation of China (31070215 and 31100181);the State Key Basic Research and Development Program of China (2009CB118504)

摘　　要：The subunit Ⅱ of chloroplast ATP synthase is one of the two peripheral stalks, which associates the catalytic CF1 with mem-brane-spanning CFo . Although the structural and functional roles of chloroplast ATP synthase have been extensively examined, the physiological significance of subunit Ⅱ in vivo is still unclear. In this work, we identified one Arabidopsis T-DNA insertion mutant of atpG gene encoding the subunit Ⅱ of chloroplast ATP synthase. The atpg null mutant displayed an albino lethal pheno-type, as it could not grow photoautotrophically. Transmission electron microscopy analysis showed that chloroplasts of atpg lacked the organized thylakoid membranes. Loss of subunit Ⅱ affected the accumulation of CF1-CFo complex, however, it did not seem to have an effect on the CF1 assembly. The light induced ATP formation of atpg was significantly reduced compared with the wild type. Based on these results, we suggested that ATPG was essential for the accumulation and function of chloroplast ATP synthase.The subunit II of chloroplast ATP synthase is one of the two peripheral stalks, which associates the catalytic CF1 with mem- brane-spanning CFo. Although the structural and functional roles of chloroplast ATP synthase have been extensively examined, the physiological significance of subunit II in vivo is still unclear. In this work, we identified one Arabidopsis T-DNA insertion mutant of atpG gene encoding the subunit II of chloroplast ATP synthase. The atpg null mutant displayed an albino lethal pheno- type, as it could not grow photoautotrophically. Transmission electron microscopy analysis showed that chloroplasts of atpg lacked the organized thylakoid membranes. Loss of subunit II affected the accumulation of CFI-CFo complex, however, it did not seem to have an effect on the CF1 assembly. The light induced ATP formation of atpg was significantly reduced compared with the wild type. Based on these results, we suggested that ATPG was essential for the accumulation and function of chloroplast ATP synthase.

关 键 词：ATP合酶 ATPG 叶绿体 拟南芥 积累 透射电子显微镜分析 T-DNA插入 类囊体膜 

分 类 号：Q946[生物学—植物学]