DNA polymerase beta overexpression correlates with poor prognosis in esophageal cancer patients  

作　　者：ZHENG Hong XUE Peng LI Min ZHAO JiMin DONG ZiMing ZHAO GuoQiang 

机构地区：[1]Department of Pathophysiology, Medical College of Henan University [2]School of Basic Medical Sciences, Zhengzhou University

出　　处：《Chinese Science Bulletin》2013年第26期3274-3279,共6页

基　　金：supported by the National Natural Science Foundation of China (39870287 and 81100759);Natural Science Foundation of Henan University (2010ZRZD02)

摘　　要：Gene of DNA polymerase beta (pol ) plays an important role in base excision repair, DNA replication and translesion synthesis. This study aims to investigate the expression and prognostic significance of DNA pol in esophageal cancer. DNA pol expression was analyzed using real-time quantitative PCR (RT-qPCR) and immunohistochemical staining on tissue samples from a consecutive series of 114 esophageal squamous carcinoma patients who underwent resections between 2002 and 2006. Pol expression was investigated on its correlation to clinico-pathological factors and survival. RT-qPCR results showed higher expression of DNA pol mRNA in tumor tissue than in its matched adjacent non-tumor tissue sample, different expression of DNA pol mRNA was noticed with significance between tumors with and without lymph node metastasis. Immunohistochemistry staining results indicated the pol strong-positive rate was 44.73% (51/114) in tumor tissue samples and 0.00% in matched adjacent non-tumor tissue samples, with significant difference. Kaplan-Meier survival curves revealed that high expression of pol was associated with tumor metastasis and poor prognosis in esophageal cancer patients. Our data suggests that pol plays an important role in tumor progression and that high pol expression predicts an unfavorable prognosis in esophageal squamous carcinoma patients.Gene of DNA polymerase beta （polβ） plays an important role in base excision repair, DNA replication and translesion synthesis. This study aims to investigate the expression and prognostic significance of DNA polβ in esophageal cancer. DNA poll expres- sion was analyzed using real-time quantitative PCR （RT-qPCR） and immunohistochemical staining on tissue samples from a consecutive series of 114 esophageal squamous carcinoma patients who underwent resections between 2002 and 2006. Polβ expression was investigated on its correlation to clinico-pathological factors and survival. RT-qPCR results showed higher expres- sion of DNA polβ mRNA in tumor tissue than in its matched adjacent non-tumor tissue sample, different expression of DNA polβ mRNA was noticed with significance between tumors with and without lymph node metastasis. Immunohistochemistry staining results indicated the polβ strong-positive rate was 44.73% （51/114） in tumor tissue samples and 0.00% in matched adjacent non-tumor tissue samples, with significant difference. Kaplan-Meier survival curves revealed that high expression of polβ was associated with tumor metastasis and poor prognosis in esophageal cancer patients. Our data suggests that poll plays an important role in tumor progression and that high polβ expression predicts an unfavorable prognosis in esophageal squamous carcinoma patients.

关 键 词：DNA聚合酶Β 食管癌 预后 患者 过表达 免疫组化染色 肿瘤组织 鳞状细胞癌 

分 类 号：R735.1[医药卫生—肿瘤]