EGF对用或未用MNNG诱导的日本血吸虫成虫培养细胞生长的影响  

The function of EGF on the proliferation of cultured Schistosoma japonicum cells treated with or without MNNG

作　　者：董惠芬[1] 蒋明森[1] 明珍平[1] 钟沁萍 杨明义[1] 

机构地区：[1]湖北医科大学寄生虫学教研室血吸虫病研究室，湖北 武汉 430071

出　　处：《中国地方病学杂志》2000年第5期347-349,共3页Chinese Jouranl of Endemiology

基　　金：湖北省自然科学基金(97J075);湖北省教委青年基金;武汉市青年科技晨光计划资助项目(985708235)

摘　　要：目的　研究表皮生长因子 (EGF)对用或未用甲基硝基亚硝基胍 (MNNG)诱导的日本血吸虫成虫培养细胞的促生长、增殖作用。方法　将联合法接种培养至第 3天的日本血吸虫成虫培养细胞 ,一部分在含 EGF终浓度分别为 0、0 .5、1、2、4、8、12、16、2 0、2 4、2 8ng/ml的附加 2 0 %小牛血清及常量抗菌素的 RPMI- 16 40培养基中培养 ;另一部分 ,先用含 MNNG终浓度为 3μg/m l附加 2 0 %小牛血清和常量抗生素的 RPMI- 16 40培养基处理48h,再换用含终浓度分别为 0、1、8、12、16、2 0 ng/ml的 EGF培养基继续培养。每日用 Olym pus IM倒置显微镜观察细胞生长和增殖情况。结果　两实验组中 ,用不同浓度 EGF处理后的日本血吸虫成虫培养细胞均在 2周以后出现不同程度的脱落、退化 ;并且 ,随着 EGF浓度的升高 ,培养细胞脱落、退化的现象更加严重 ;两实验组相比 ,未用 MNNG诱导的培养细胞出现脱落和退化现象的时间比用 MNNG诱导的相对更早。结论　外加 EGF对用或未用 MNNG诱导的日本血吸虫成虫培养细胞的生长增殖均无促进作用 ,相反 ,加速了培养细胞的老化 ,尤其是对未用 MNNG诱导的日本血吸虫成虫培养细胞 。Objective To study the effects of EGF on the growth and proliferation of cultured cells from adult Schistosoma japonicum.Methods Cells collected from the adult Schistosoma japonicum were cultured in medium RPMI 1640 containing 20% calf serum and a moderate amount of antibiotics for 3 days,they were divided into two groups.The cells in the first group were directly treated by EGF with concentrations of 0,0.5,1,2,,4,8,12,16,20,24 and 28 ng/ml,respectively.The cells in the second group were designed to treat by MNNG of 3 μg/ml for 48 hours before they were cultured in the medium with EGF of 1,8,12,16 and 20 ng/ml,respectively.The growth and proliferation of cultured cells were observed by microscope everyday.Results Some of the cultured cells in the medium with different concentration of EGF fell off and degenerated in both groups after two weeks.Moreover,along with the increase of the EGF concentration in the medium,the abscission and degeneration of the cells became serious.Cultured cells in the first group fell off or degenerated earlier than those in the second group.Conclusions EGF cannot induce the proliferation of the cultured cells from adult Schistosoma japonicum.On the contrary,it can accelerate the degeneration of the cultured cells,especially for the cells not treated by MNNG.

关 键 词：日本血吸虫 表皮生长因子 MNNG 成虫培养细胞 

分 类 号：R532.21[医药卫生—内科学] R383.24[医药卫生—临床医学]