siRNA下调LSD1基因对卵巢癌OVCAR-3细胞增殖和周期的影响  

The effects of LSD1 gene down-regulation induced by siRNA on the proliferation and cell cycle of ovarian cancer cell line OVCAR-3

作　　者：孙曼[1] 生秀杰[1] 刘启才[2] 李祯[1] 汪志辉[1] 

机构地区：[1]广州医学院第三附属医院妇产科,广州510000 [2]广州医学院实验医学研究中心,广州510000

出　　处：《癌症进展》2013年第3期229-234,共6页Oncology Progress

基　　金：广州市科技计划资助项目(2010GN-E00221)

摘　　要：目的应用小分子干扰RNA(small interfering RNA,siRNA)抑制赖氨酸特异性组蛋白去甲基化酶1(lysine specific demethylase 1,LSD1)基因的表达,探讨其对卵巢癌OVCAR-3细胞增殖和生长周期的影响。方法采用脂质体介导法将LSD1-siRNA转染卵巢癌OVCAR-3细胞;实时荧光定量PCR(RFQ-PCR)和Western blot法检测基因的表达;MTT法检测细胞增殖能力;FCM分析细胞周期。结果成功转染LSD1-siRNA后,OVCAR-3细胞中LSD1 mRNA和蛋白表达均明显下降;细胞增殖明显抑制;G_2/M期细胞比例增加。结论 LSD1-siRNA可显著下调LSD1基因在卵巢癌OVCAR-3细胞中的表达水平,在一定程度上抑制卵巢癌细胞的增殖,导致细胞周期阻滞。Objective To study the effect of down-regulation of lysine specific demethylase 1 （ LSD1 ） induced by the siRNA on the proliferation and cell cycle of human owtrian cancer cell line OVCAR-3. Method The siRNA was trans- fected into ovarian cancer OVCAR-3 cells by LipofectAMINE2000. The expression of LSD1 mRNA and protein were exam- ined by real time fluorescence quantitative PCR （RFQ-PCR） and Western blot. The MTF method and flow cytometly were applied to detect the proliferation ability and cell cycle distribution of OVCAR-3 cells. Result The expressions of LSD1 mRNA and proteins were all decreased markedly after transfection with LSDI-siRNA; the growth of OVCAR-3 cells was significantly inhibited; the percentage of cells in G2/1M phase became higher. Conclusion LSDI-siRNA can down-regu- late LSD1 gene expression in human ovarian cancer OVCAR-3 cells, suppress cell proliferation to some extent and induce cell cycle arrest.

关 键 词：卵巢癌 小分子干扰RNA 细胞增殖 LSD1 OVCAR-3细胞 

分 类 号：R737.31[医药卫生—肿瘤]