CpG寡核苷酸对C57BL/6小鼠肝癌模型影响的初步研究  

Effects of CpG oligonucleotides on C57BL/6 mouse model of hepatocellular carcinoma

作　　者：司小刚[1] 罗峰[1] 黄玲[1] 

机构地区：[1]井冈山大学附属医院胸外科,江西吉安343000

出　　处：《中国医学工程》2013年第6期16-17,共2页China Medical Engineering

摘　　要：目的建立C57BL/6小鼠肝癌模型并研究CpG寡核苷酸(ODN)对C57BL/6小鼠肝癌模型的治疗效果。方法用HE染色鉴定C57BL/6小鼠肝癌模型。肝癌模型建立后,分别用PBS及CpG寡核苷酸处理C57BL/6小鼠肝癌模型,并用FCM检测小鼠脾脏CD3-FITC、CD4-FITC、CD8-FITC、NK1.1-FITC的组成,ELISA法测免疫小鼠血清中IFN-γ、IL-4水平。结果成功建立C57BL/6小鼠肝癌模型,ODN处理组的肝癌细胞裂解物中CD3-FITC、CD4-FITC、CD8-FITC、NK1.1-FITC的体外杀伤活性以及荷瘤小鼠血清中IFN-γ、IL-4水平均显著高于PBS组。结论 CpG ODN能够提高小鼠对肝癌的免疫功能。[ Objective ] To establish of C57BL / 6 mouse hepatoma model and CpG oligonucleotide （ODN） treatment of C57BL / 6 mouse liver cancer model. [ Methods ] HE staining identification of C57BL/6 mouse liver cancer model. After the liver cancer model, respectively, with PBS and CpG ODN to deal with the C57BL/6 mouse liver cancer model, and the composition of FCM analysis of murine splenic CD3-FITC, measure the immune serum IFN-γ, IL-4 level [ Reslnts ] sue CD4-FITC, CD8-FITC, of NK1.1-FITC, ELISA method to cessfully established in the C57BL / 6 mouse liver cancer model, ODN treated group of hepatoma cell lysates CD3-FITC, CD4-FITC, CD8-FITC, of NK1.1-FITC in vitro cytotoxic activity of tumor-bearing mice serum IFN-γ, IL-4 water were significantly higher than the PBS group. [ Conclusion ] CpG ODN can enhance the immune function of mice liver cancer, especially the specificity of cellular immune function.

关 键 词：肝癌模型 ODN 细胞免疫 

分 类 号：R735.7[医药卫生—肿瘤]