非编码小RNA分子miR-34a在乳腺癌细胞系中的表达及意义  

Expression and its significance for non-coding small RNA molecule miR-34a in breast cancer cell lines

作　　者：杨璐全[1] 龙燕[1] 魏春莉[1] 杨维婵[1] 张连美[1] 杨曼曼[1] 张天丹[1] 傅俊江[1,2] 

机构地区：[1]泸州医学院医学基础研究中心表观遗传与肿瘤重点实验室,四川泸州646000 [2]Department of Molecular and Cellular Biology, Baylor College of Medicine,One Baylor Plaza

出　　处：《国际病理科学与临床杂志》2013年第4期292-295,共4页Journal of International Pathology and Clinical Medicine

基　　金：国家自然科学基金(81172049;30371493);四川省高校创新团队(13TD0032)~~

摘　　要：目的:比较各种不同类型的乳腺癌细胞系miR-34a的表达,探索作为抑癌基因的miR-34a在抑制肿瘤转移中的临床意义。方法:用micro RNA特异性试剂盒提取总RNA,应用特异性茎-环反转录实时PCR技术检测miR-34a在各种乳腺癌细胞中的表达,并进行比较分析。结果:提取的RNA质量完好,miR-34a在所检测的高侵袭性乳腺癌细胞MDA-MB-231,MDA-MB-435和BT-549中呈低表达;而在低侵袭性细胞MCF7和T47D中呈相对高表达。结论:建立了特异性茎-环反转录实时PCR检测非编码小RNA分子技术;miR-34a的表达与肿瘤细胞高侵袭性可能呈负相关,提示miR-34a作为抑癌基因可能在抑制肿瘤转移中起重要作用,在乳腺癌诊断、预防和治疗中具有重要的潜在价值。Objective： To compare the miR-34a expression files in different types of breast cancer cell lines, and to explore the clinical significance for suppressing tumor metastasis. Methods： The total RNAs, including small non-coding RNA were extracted by using miRNeasy mini kit and specific stem-loop reverse transcription real-time PCR was performed and analyzed for detecting miR-34a expression in cell lines of breast cancer. Results： The high quality total RNAs were attained. The expression level of miR-34a was low in breast tumor cells, MDA-MB-231, MDA-MB-435, and BT-549, with high aggressiveness, whereas the expression level of miR-34a was high in breast tumor cells, MCF7 and T47D, with high aggressiveness. Conclusion： We successfully establish specific stem-loop reverse transcription real-time PCR for detecting small non-coding RNA. The expression level of miR-34a may be negative correlated with tumor cells’ high aggressiveness, suggesting that as a tumor suppressor, miR-34a plays an important role in suppressing tumor metastasis, and has great potential for breast cancer diagnosis, prevention, and therapy.

关 键 词：MIR-34A 乳腺癌 细胞系 表达 肿瘤转移 

分 类 号：R737.9[医药卫生—肿瘤]