高分辨率熔解曲线分析应用的研究进展  

Research Progress on the Application of High-resolution Melting Curve Analysis

作　　者：刘自增[1] 吴周良 阎萍[2] 王一文[1] 

机构地区：[1]天津市奶牛发展中心,天津300384 [2]中国农业科学院兰州畜牧与兽药研究所畜牧研究室,甘肃兰州730050

出　　处：《中国畜牧兽医》2013年第8期105-111,共7页China Animal Husbandry & Veterinary Medicine

基　　金：国家肉牛牦牛产业技术体系(CARS-38);青年科学基金项目(31101702)

摘　　要：高分辨率熔解曲线分析(high-resolution melting curve analysis,HRMA)是结合饱和荧光染料、未标记探针和实时荧光定量PCR的一种新的检测基因突变与基因分型的分子诊断技术,具有高通量、低成本、简单快捷、结果准确、灵敏度和特异性高及真正闭管操作等优点。作者就高分辨率熔解曲线分析应用的研究进展进行综述。Abstract.. High resolution melting analysis （HRMA） was a new molecular diagnosis method for mutation scanning and genotyping, which combined special saturated fluorescent dyes, unlabled probe and Real-time PCR. High resolution melting analysis was sfiperior to other nucleic acid research methods, including an inexpensive closed tube format that was a rapid, accurate, sensitive, special and high-throughput assay. In this paper, advancement on the application of high resolution melting in molecular diagnosis was summarized.

关 键 词：突变检测 熔解曲线分析 核酸 

分 类 号：S816.79[农业科学—饲料科学]