LRP16基因在子宫内膜癌组织中的表达及其对细胞增殖的作用  

Expression of LRP16 in human endometrial carcinoma tissues and its role on cell proliferation

作　　者：张宇骄[1] 刘玲[1] 汪春燕[1] 詹平[1] 伍宗惠[1] 何雯[1] 王定玉[1] 

机构地区：[1]泸州医学院附属医院妇产科,四川泸州646000

出　　处：《重庆医学》2013年第25期2983-2985,共3页Chongqing medicine

基　　金：四川省泸州医学院课题资助项目(07055)

摘　　要：目的研究LRP16在子宫内膜癌组织中的表达变化及其对子宫内膜癌HEC-1-B细胞增殖的影响。方法应用逆转录聚合酶链反应(RT-PCR)检测26例正常子宫内膜组织及10例子宫内膜癌组织中LRP16mRNA的表达;LRP16转染细胞,RT-PCR证实转染的成功性,WST-1法观察细胞增殖的变化。结果子宫内膜癌组织中LRP16mRNA的阳性表达率及表达水平(分别为83.33%、0.82±0.21)明显高于正常子宫内膜组织(分别为30.00%、0.47±0.18),二者比较差异有统计学意义(P<0.05)。RT-PCR检测结果显示转染后细胞LRP16mRNA表达明显增加。转染组HEC-1-B细胞在体外能继续增殖,但增殖能力并没有增强。结论 LRP16的异常表达可能与子宫内膜癌的发生、发展密切相关,LRP16基因用于子宫内膜癌基因治疗可能具有潜在价值。Objective To investigate the expression change of LRP16 in endometrial cancer tissues and its influence on the pro- liferation of human endometrial carcinoma HEC-1-B cells. Methods HEC-1-B cells were transfected with LRP16. RT-PCR was used to examine the expression of LRP16 in 26 normal endometrium specimens, 10 endometrial cancer specimens. RT-PCR was used for verifying the transfection success. WES-T was used to observe the proliferation change of HEC-1-B cells. Results The positive expression rate and level of LRP16 mRNA in the endometrial cancer tissues were 83.33 % and 0. 82±0. 21, which were significantly higher than 30.00% ,0.47±0.18 in the normal endometrium tissues（P〈0.05）. The RT-PCR detection results revealed that the expression of LRP16 mRNA after transfeetion was significantly increased. HECq-B cells in the transfection group could continued to proliferate in vitro,but the proliferation capacity was not increased. Conclusion The expression abnormality of LRP16 may be closely related to the occurrence and progress of endometrial cancer, LRP16 gene may have potential value for the endometrial cancer gene therapy.

关 键 词：子宫内膜肿瘤 LRP16基因 HEC-1-B细胞 

分 类 号：R737.33[医药卫生—肿瘤]