兽用抗生素胁迫对空心菜的影响研究  

Effects of veterinary antibiotics stress on Ipomoea aquatica Forsk

作　　者：廖德润[1] 刘超翔[1] 王振[1] 刘琳[1] 刘玉洪[1] 郑加玉[1] 林国徐 方静 

机构地区：[1]中国科学院城市环境研究所,厦门361021 [2]福建省莆田市优利可农牧发展有限公司,莆田351115 [3]福建省莆田市涵江区江口畜牧兽医站,莆田351115

出　　处：《环境科学学报》2013年第9期2558-2564,共7页Acta Scientiae Circumstantiae

基　　金：福建省科技计划重点项目(No.2012Y0068);福建省自然科学基金面上项目(No.2012J01241);国家重点实验室专项基金(No.12K06ESPCT);福建省星火计划重点项目(No.2011S0011);莆田市科技计划院地合作项目(No.2012S15)~~

摘　　要：考察了土霉素(OTC)、磺胺二甲基嘧啶(SM2)和环丙沙星(CIP)3种兽用抗生素对空心菜(Ipomoea aquatica Forsk)根系活力、叶片叶绿素含量和抗氧化酶活性等生理特性的影响,并分析了抗生素在空心菜体内的累积情况.试验结果表明,较低浓度抗生素(<1.0μg·L-1)对空心菜的生长具有刺激效应;较高浓度抗生素(>50μg·L-1)对空心菜的生长则具有抑制作用.当抗生素浓度大于1.0μg·L-1时,空心菜的根系活力受到抑制;当抗生素浓度大于50μg·L-1时,空心菜的叶片叶绿素含量及抗氧化酶活性均受到抑制;当抗生素浓度大于500μg·L-1时,空心菜体内的蛋白质含量减少.另外,OTC、SM2和CIP3种抗生素在空心菜内的累积量不同,其累积量依次为CIP>SM2>OTC.抗生素在空心菜内的分布也存在较大差异,根部3种抗生素的含量均明显高于茎和叶片.A hydroponic experiment was carried out to investigate the effects of veterinary antibiotics, i.e. oxytetracycline （OTC）, sulfamethazine （SM2） and ciprofloxacin（CIP）, on chlorophyll contents, root activity, and antioxdative enzymes as well as the accumulation of antibiotics in Ipomoea aquatica Forsk. The results showed that, lower antibiotics concentrations （ 1.0 μg · L-1） had a stimulatory effect on the growth of I. aquatica Forsk, whereas higher antibiotics concentrations （ 50 μg · L-1） had an inhibitory effect. The root activity of Ipomoea aquatica Forsk was inhibited when the antibiotics concentrations were greater than 1.0μg · L-1, the chlorophyll contents and antioxdative enzymes were inhibited when the antibiotics concentrations were greater than 50 μg · L-1, and the protein content in Ipomoea aquatica Forsk significantly decreased when the antibiotics concentrations were greater than 500 μg · L-1. In addition, the cumulants of the three antibiotics were different from each other in the I. aquatica Forsk. The CIP cumulant was the highest in the plant, followed by SM2 and OTC. For each antibiotics, the cumulants in different parts of plant varied significantly, and the cumulant of each antibiotics in root was much higher than in stem and leaf.

关 键 词：空心菜 抗生素 累积 胁迫反应 分配 

分 类 号：X171.5[环境科学与工程—环境科学]