发酵萃取耦合工艺生产丁醇条件的优化  

Optimization of conditions of extraction fermentation process for butanol production

作　　者：王鑫昕[1] 叶嘉[1] 耿霄[1] 曹丽君[2] 王少华[3] 张延平[3] 

机构地区：[1]邯郸学院生物科学系,河北邯郸056005 [2]唐山师范学院,河北唐山063000 [3]中国科学院微生物研究所,北京100101

出　　处：《河北农业大学学报》2013年第4期76-81,共6页Journal of Hebei Agricultural University

基　　金：国家高技术研究发展计划(863计划)(2006AA02Z237)

摘　　要：传统丙酮丁醇发酵的产物抑制作用造成丁醇产量较低,本文选择发酵萃取耦合工艺将丁醇原位分离出来,以降低抑制作用。通过冷模试验、生长影响试验和萃取发酵试验,确定了有机相条件和发酵萃取耦合工艺:有机萃取相为油醇中加入20%癸醇,相比为1∶5(有机相∶水相),发酵24h后将有机相导入发酵体系中。利用该工艺,丁醇和总溶剂产量分别比传统发酵的相应产量提高了23.99%和13.33%。Acetone-butanol-ethanol fermentation is significantly inhibited by high concentration of butanol. In this paper, extractive fermentation was used to remove butanol in situ from the fermentation process. By detecting butanol extraction efficiency with or without cells and the effect on cell growth, components of extraction phase were optimized, with 80% oleyl alcohol mixed with 20% decanol alcohol. By adding the organic phase of 20% volume of fermentation broth into the fermentation system at 24 h, final titers of butanol and total solvents reached 23.99% and 13.33% of those of the control, respectively.

关 键 词：丁醇 发酵萃取 丙酮丁醇发酵 油醇 癸醇 原位分离 

分 类 号：Q815[生物学—生物工程]