白介素-9在鼠Ⅲ型肝炎病毒诱导的暴发性肝炎小鼠肝脏自然杀伤细胞及自然杀伤T细胞的表达研究  

Increased percentage of hepatic IL-9-secreting NK and NKT cells in MHV-3-induced fulminant hepatitis in mice

作　　者：丁琳[1] 陈韬[1] 师爱超[1] 宁琴[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院感染性疾病研究所感染病科,湖北武汉430030

出　　处：《中西医结合肝病杂志》2013年第4期214-216,共3页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

基　　金：国家自然科学基金面上项目(No.NSFC81171558);青年科学基金项目(No.81100308)

摘　　要：目的:研究MHV-3(鼠Ⅲ型肝炎病毒)感染所致的暴发性肝炎小鼠模型中,IL(白细胞介素)-9在肝脏NK(自然杀伤细胞)及NKT细胞的表达变化,及其与肝损伤之间的关系。方法:BALB/cJ小鼠腹腔注射100pfu(空斑形成单位)MHV-3建立小鼠暴发性肝炎模型,应用流式细胞术荧光分析法检测MHV-3感染0小时、24小时、48小时和72小时肝脏NK及NKT细胞表达IL-9的比例变化,并检测小鼠血清中丙氨酸转氨酶(ALT)水平。结果:BALB/cJ小鼠感染MHV-3后,IL-9在肝脏NK细胞的表达在感染24小时显著升高(P<0.05),并于48小时达最高水平(P<0.05);在肝脏NKT细胞的表达于感染24小时显著升高(P<0.05),并于48小时达最高水平,且均与肝损伤指标(血清ALT水平)呈正相关。结论:随着感染时间延长,肝脏分泌IL-9的NK和NKT淋巴细胞显著升高,提示IL-9参与了MHV-3诱导的暴发性肝炎小鼠免疫诱导的肝脏损伤发病过程。Objective： To investigate the changes of hepatic IL-9-secreting NK and NKT cells in MHV-3-induced fulminant hepatitis in mice and their correlation with liver injury. Methods： MHV-3-induced fulminant hepatitis model was established in mice. The percentage of hepatic IL-9-secreting NK and NKT cells were determined by flow cytometry, and the level of ALT in se- rum were examined. Results： By the time-colirse of MHV-3 infection, the percentage of hepatic IL-9-producing NK cells in- creased at 24h （ P 〈 0.05 ） and reached peak at 48h with great significance, while the percentage of hepatic IL-9-producing NKT cells increased at 24h （P 〈 0.05 ） and reached peak at 48h with no significance. The percentage of hepatic IL-9-producing NK and NKT cells were both positive correlated to the index of liver injury （serum ALT） . Conclusion： The increased expression of IL-9 from hepatic NK and NKT lymphocytes may contribute to immune-mediated liver injury in MHV-3-induced fulminant hepati- tis in mice.

关 键 词：鼠Ⅲ型肝炎病毒 暴发性肝炎 白细胞介素-9 自然杀伤细胞 自然杀伤T细胞 

分 类 号：R575.1[医药卫生—消化系统]