穿心莲内酯对胶质瘤U87细胞增殖的抑制作用及其机制  

Inhibitory effect of andrographolide on cell proliferation of glioblastoma U87 cells and its mechanism

作　　者：章倩倩[1] 丁一[1] 亓翠玲[1] 兰天[1] 李江超[1] 何晓东[1] 王丽京[1] 

机构地区：[1]广东药学院血管生物学研究所,广东广州510006

出　　处：《吉林大学学报（医学版）》2013年第4期676-679,共4页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(31200896);广东省自然科学基金资助课题(S2012040007658);广东省医学科研基金资助课题(B2012181)

摘　　要：目的:观察穿心莲内酯(Andro)对胶质瘤U87细胞增殖的抑制作用,阐明其作用机制。方法:取对数生长期U87细胞,分为二甲基亚砜(DMSO)对照组、4H-穿心莲内酯(4H-Andro)对照组和Andro组,MTT法检测不同浓度(0、5、10、15、20和25μmol.L-1)DMSO、4H-Andro及Andro对U87细胞活力的影响,计算U87细胞半数抑制浓度(IC50);Western blotting法检测各组U87细胞NF-κB蛋白表达强度。结果:通过浓度筛选,Andro平均IC50为8μmol.L-1。与DMSO和4H-Andro对照组比较,8μmol.L-1 Andro处理24h后U87细胞增殖活性降低(P<0.01),增殖抑制率达到50%;Andro处理96h时,U87细胞增殖活性进一步降低(P<0.01),增殖抑制率达到65%。与DMSO和4H-Andro对照组比较,8μmol.L-1 Andro处理48h后,U87细胞中NF-κB(即磷酸化p65)蛋白表达强度下降(P<0.01)。结论:Andro作为临床上常用的天然抗炎药物,具有抑制胶质瘤细胞增殖的功能,并且可通过靶向抑制NF-κB蛋白表达强度发挥作用。Objective To observe the inhibitory effect of andrographolide（Andro）on cell proliferation of glioblastoma U87cells,and to explore its regulatory mechanism.Methods The U87cells at logarithmic growth phase were collected and divided into dimethy1sulfoxide（DMSO）control group,4H-Andro control group and Andro group.The U87cells were treated with DMSO,4H-Andro,and Andro with different concentrations（0,5,10,15,20,25μmol.L-1）.The cell viability was detected by MTT assay,and the mean inhibitory concentraion（IC50）was caculated,and Western blotting method was used to detect the expression intensity of NF-κB protein in U87cells.Results The U87cells were treated with different concentrations of Andro,and the IC50of Andro was 8μmol.L-1.Compared with DMSO and 4H-Andro control groups,24hafter treatment of 8μmol.L-1 Andro,the proliferation activity of U87cells was inhibited（P0.01）and the inhibitory rate of proliferation was 50%;and the cell proliferation was obviously decreased after treated with Andro for 96h（P 0.01）in U87cells,and the inhibitory rate of proliferation was 65%.The expression intensity of NF-κB（pp65） protein was down-regulated by 8μmol.L-1 Andro for 48hcompared with DMSO control group and 4H-Andro control group（P0.01）.Conclusion Andro,as an anti-inflammatory drug in clinic,plays a new function of suppressing glioblastoma cell proliferation through inhibiting the expression intensity of NF-κB protein.

关 键 词：穿心莲内酯 胶质瘤细胞 细胞增殖 

分 类 号：R73-36[医药卫生—肿瘤]