HIF-1α和RelA基因在急进高原正常人外周血单个核细胞中的表达及其与急性高原反应的关联性  

Expressions of HIF-1α and RelA in mononuclear cells in peripheral blood of health adults after ascentting of plateau and their correlations with pathogenesis of acute mountain sickness

作　　者：方盼盼[1,2] 宫璀璀[1,2] 仓宝成[1,2] 李正民[1,2] 李宗杰[1,2] 李文莉[2] 朱传杰[2] 王迎涛[2] 

机构地区：[1]郑州大学第三附属医院科研中心,河南郑州450052 [2]中国人民解放军153中心医院中心实验室,河南郑州450042

出　　处：《吉林大学学报（医学版）》2013年第4期781-785,共5页Journal of Jilin University：Medicine Edition

基　　金：河南省科技厅基础与前沿技术研究计划项目资助课题(072300410440)

摘　　要：目的:观察急进高原前后正常人单个核细胞中缺氧诱导因子1α(HIF-1α)和核转录因子NF-κB p65亚基(RelA)表达水平的变化,探讨HIF-1α和RelA在急性高原反应(AMS)发生中的作用及机制。方法:对某部赴玉树抗震的士兵分别于进入高原前和进入高原后48h采集外周血,提取单个核细胞中的mRNA和蛋白,采用RT-PCR方法检测RelA和HIF-1αmRNA表达水平,Western blotting法检测HIF-1α蛋白表达水平。Spearman相关分析法分析RelA和HIF-1α基因表达与AMS发生的相关性。结果:与急进高原前比较,进入高原后HIF-1α和RelA mRNA及HIF-1α蛋白表达水平均显著增高(P<0.01);HIF-1αmRNA和RelA mRNA表达呈正相关关系(r=0.806,P<0.01);HIF-1α蛋白表达与AMS的发生率呈正相关关系(r=0.875,P<0.01);HIF-1α和RelA表达不同性别之间比较差异无统计学意义(P>0.05)。结论:HIF-1α蛋白表达水平可作为预测和干预AMS的监测指标。急进高原后HIF-1α和RelA mRNA表达水平显著增加且呈显著正相关关系,提示在其信号传导通路之间存在cross-talk现象。Objective To observe the changes of expression levels of hypoxia-inducible factor 1alpha（HIF-1α）and nuclear factor kappa B（NF-κB）p65（RelA）in mononuclear cells after ascenting of plateau and their correlations with the incidence rate of acute mountain sickness（AMS）,and to explore the role and mechanism of HIF-1αand RelA in pathogenesis of AMS.Methods The soldiers’venous blood before ascentting of plateau and 48hafter arriving Yushu were collected,and the protein and mRNA in mononuclear cells were extracted.The expressions of HIF-1αand RelA mRNA were determined by RT-PCR.The expression of HIF-1αprotein was determined by Western blotting method.The correlations between the expressions of HIF-1αand RelA and pathogenesis of AMS were analyzed with Spearman analysis.Results Compared with before ascentting,after ascentting of plateau the expression levels of HIF-1αmRNA,RelA mRNA and HIF-1αprotein were increased significantly（P0.01）.The expression of HIF-1αmRNA was positively correlated with the RelA mRNA expression（r=0.806,P0.01）.At the same time,the expression of HIF-1αprotein was positively correlated with the pathogenesis of AMS（r= 0.875,P0.01）;and the expression levels of HIF-1αand RelA had no significant difference between women and men（P0.05）.Conclusion The expression level of HIF-1αprotein is increased significantly after asentting of plateau,and has significantly positive correlation with the pathogenesis of AMS.The expression levels of HIF-1α and RelA mRNA are significantly increased 48hafter ascentting of plateau,and they have positive correlation,prompting that the existence of cross-talk phenomenon between their signal transduction pathways.

关 键 词：急性高原反应 缺氧诱导因子1α 核转录因子KAPPAB p65亚基 缺氧 CROSS-TALK 

分 类 号：R394.3[医药卫生—医学遗传学]