响应面法优化连翘中桦木酸超声辅助提取工艺  

Optimization of Ultrasound-Assisted Extraction Technology for Betulinic Acid from Forsythiae Fructus by Response Surface Methodology

作　　者：张生[1] 王宇[1] 王少明[1] 魏焕勇[1] 郭盛磊[1] 阎秀峰[1] 

机构地区：[1]东北林业大学盐碱地生物资源环境研究中心东北油田盐碱植被恢复与重建教育部重点实验室,哈尔滨150040

出　　处：《中国实验方剂学杂志》2013年第17期15-18,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：林业公益性行业科研专项(201004069);浙江省自然科学基金重点项目(Z2100435);黑龙江省自然科学基金项目(C201101)

摘　　要：目的:应用响应面法优化超声辅助提取连翘中桦木酸的工艺条件。方法:在单因素试验基础上,采用Box-Behnken试验设计和响应面分析法考察乙醇体积分数、提取温度、液固比对桦木酸提取率的影响。HPLC测定桦木酸含量,Thermo Hypersil GOLD AQ-C18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-水(9∶1),流速1.2 mL·min-1,柱温25℃,进样量20μL,检测波长210 nm。结果:最佳超声提取工艺为乙醇体积分数80%,提取温度37℃,液固比32 mL.g-1,桦木酸提取率(4.28±0.05)mg·g-1,与模型预测值(4.30 mg·g-1)基本一致。结论:该工艺合理、可靠,有效成分提取率高。Objective：To optimize ultrasonic-assisted extraction technology of betulinic acid from Forsythiae Fructus using response surface methodology.Method：On the basis of single factor tests,Box-Behnken experimental design and response surface methodology was adopted to optimize extraction conditions with the concentration of ethanol,extraction temperature and solid-liquid ratio as factors.HPLC was used to determine the content of betulinic acid from Forsythiae Fructus,chromatographic conditions were as follows：Thermo Hypersil GOLD AQ-C18 column（4.6 mm × 250 mm,5 μm）,acetonitrile-water（9：1） as mobile phase,flow rate of 1.2 mL·min-1,injection volume 20 μL,detection wavelength at 210 nm.Result：Optimal ultrasound-assisted extraction technology of betulinic acid from Forsythiae Fructus was as following：the volume fraction of ethanol 80%,extraction temperature 37 ℃,liquid-solid ratio 32 mL.g-1.The actual value of betulinic acid yield was（4.28 ± 0.05） mg·g-1,which was consistent with the predicted value of 4.30 mg·g-1.Conclusion：This optimized extraction process was reasonable and reliable with high yield of effective components.

关 键 词：连翘 桦木酸 超声辅助提取 单因素试验 

分 类 号：R283.6[医药卫生—中药学] R284.2[医药卫生—中医学]