MALT1-A20-NF-κB信号分子在MM中的表达特点  

Expression feature of MALT1,A20 and NF-κB in MM

作　　者：王旭[1] 徐玲[2] 张帆[2] 徐艳[1] 吴秀丽[2] 杨力建[2] 陈少华[2] 李萡[2] 卢育洪[3] 李扬秋[1,2] 

机构地区：[1]暨南大学再生医学教育部重点实验室,广东广州510632 [2]暨南大学血液病研究所,广东广州510632 [3]暨南大学第一附属医院血液科,广东广州510632

出　　处：《暨南大学学报（自然科学与医学版）》2013年第4期373-377,共5页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：国家自然科学基金(91129720);广东省科技计划(国际合作)项目(2012B050600023)

摘　　要：目的:了解多发性骨髓瘤(MM)患者外周血中黏膜相关组织淋巴瘤异位基因1(MALT1)、A20与核转录因子κB(NF-κB)基因的表达特点。方法:采用SYBR GreenⅠ荧光实时定量PCR和相对定量分析法检测20例MM患者(Ⅰ期7例,Ⅱ期3例,Ⅲ期10例)及21例健康人外周血单个核细胞(PBMC)中MALT1、A20和NF-κB基因的表达情况。以β2微球蛋白基因(β2m)作为内参;采用相对定量公式:α^(-△Ct)×100%,分别计算MALT1、A20和NF-κB基因的表达水平。结果:MM患者PBMC中的MALT1和A20基因的表达水平较健康人都有明显的降低(P=0.000),而MM中NF-κB基因的表达水平较正常人有所升高,但无统计学差异(P>0.05)。在健康人组,MALT1和NF-κB的表达水平呈现正相关(P=0.001,r=0.666),而在MM组,MALT1、A20与NF-κB 3种基因均不存在明显相关性。同时,Ⅰ、Ⅱ、Ⅲ期MM患者MALT1基因的表达量较健康人明显降低(P<0.05),A20基因的表达量较健康人明显降低(P<0.01),而Ⅰ、Ⅱ、Ⅲ期MM患者MALT1、A20和NF-κB之间的表达情况均无统计学差异。结论:当MALT1-A20介导的细胞免疫和炎症的信号通路发生异常改变时,可能与MM的发生发展有着密切关系。Aim：To understand the expression feature of MALT1, A20 and NF-κB in the multiple my- eloma（MM） from peripheral blood mononuclear cell （PBMCs）. Methods： Real -time PCR with SYBR Green I technique were used to determine the expression of MALT1, A20 and NF-κB genes from PBMCs of 20 MM patients （ stage 1 7 samples; stage II 3 samples ; stage m 10 samples）. The peripheral blood from 21 cases healthy individuals was served as control. By using ~2-microglobulin gene （[32m） as an endogenous reference, relative mRNA expression level of MALT1, A20 and NF-κB genes was analyzed by using the a ^△Ct,× 100% method. Results： The relative mRNA expression level of MALT1 and A20 in PB- MCs from MM patients was significantly lower than those from healthy individuals （P = 0. 000）. However, the expression level of NF-κB gene was higher than those from healthy individuals （ P 〉 0. 05 ）, butthere is no significant difference. In addition, there was a significant positive correlation between the relative expression levels of MALT1 and NF-κB gene. However, there are no significant correlation in MALT1 ,A20 and NF-κB genes. Moreover, the expression level of MALT1 gene was significantly lower than those from healthy individuals （P 〈 0. 05 ） in stage I, stage Ⅱ and stage Ⅲ of MM. While the expression level of A20 gene was also significantly lower than those from healthy individuals （ P 〈 0. 05 ） stage I , stage Ⅱ and stage Ⅲ of MM. No significant difference expression level of MALT1, A20, and NF-κB among stage I. stage Ⅱ and stage Ⅲ of MM. curred in MALT1-A20 mediated cellular immune and development of MM. Conclusion：In MM, when abnormal changes oc- inflammatory signaling pathways, it may affect the

关 键 词：多发性骨髓瘤 多发性骨髓瘤(MM) 黏膜相关组织淋巴瘤异位基因1(MALT1) A20 NF-ΚB 基因表达 

分 类 号：R733[医药卫生—肿瘤] Q786[医药卫生—临床医学]