皂荚半乳甘露聚糖胶分级醇沉及表征  

Grading Alcohol Precipitation and Characterization of Gleditsia sinensis Lam. Galactomannan Gum

作　　者：林雪娇[1] 周自圆[1] 菅红磊[1] 章伟岸[1] 孙达峰[2] 张卫明[2] 蒋建新[1] 

机构地区：[1]北京林业大学材料科学与技术学院,北京100083 [2]南京野生植物综合利用研究院,江苏南京210042

出　　处：《中国野生植物资源》2013年第4期20-23,共4页Chinese Wild Plant Resources

基　　金：国家"十二五"科技支撑计划(2012BAD32B06;2012BAD36B01);国家自然科学基金项目(31270624);北京市自然科学基金项目(6122023)

摘　　要：使用不同浓度乙醇和异丙醇分别对皂荚半乳甘露聚糖胶水溶液进行分级沉降,沉淀物用凝胶渗透色谱(GPC)和高效液相色谱(HPLC)等进行表征。结果表明,异丙醇可在较小浓度下更快沉降皂荚多糖胶,当异丙醇溶液浓度为28.6%(V/V)时,沉淀物中半乳甘露聚糖浓度达到12.50%(w/w);随着醇浓度上升,沉降组分半乳甘露聚糖得率呈增加趋势,且在后期增加幅度最大,多糖最高得率可达80%,纯化后皂荚多糖胶(GSG)表现出较高的甘露糖/半乳糖(M/G)之比值,在异丙醇沉降中表现更加明显(低浓度的异丙醇达到最高的M/G=4.1);低浓度醇沉主要得到大分子组分,随乙醇浓度增加组分分子量明显降低,多糖胶更加均匀,而在异丙醇沉降后期均一性有所下降。Gleditsia sinensis Lain. galactomannan （GSG） was precipitated using different concentrations of ethanol and isopropanol. The sediments were then characterized by gel permeation chromatography （GPC） and high performance liquid chromatography （HPLC）. Studies have shown that isopropanol can precipitate GSG more quickly even at a relatively low concentration. Also higher concentration of isopro- panol （28.6%, V/V） leads to a high galactomannan concentration （up to 12.50%, w/w） of the sedi- ment. The galactomannan yield of sediment component has an increasing trend with increasing concentra- tion of the two alcohols with the highest yield more than 80%. Purified GSG shows a higher M/G ratio, which perform more obviously in isopropanol （ the highest M/G = 4.1 ）. Precipitation by low concentra- tion of alcohol obtains the macromolecular component. And the molecular weight decreases significantly with the concentration of alcohol, at which time the polysaccharide is more uniform. Notably, the homo- geneity of polysaccharide decreases in the isopropanol precipitation.

关 键 词：皂荚 半乳甘露聚糖 乙醇 异丙醇 分级沉降 表征 

分 类 号：TQ432.9[化学工程] Q539.6[生物学—生物化学]