几种不同方式诱导Jurkat细胞凋亡过程中TFAR19的表达  

Expression of TFAR19 in Apoptotic Processes of Jurkat Cells Induced with Various Methods

作　　者：李莉[1] 陈英玉[2] 郑蕊[1] 马大龙[2] 王德炳[1] 

机构地区：[1]北京医科大学人民医院,血液病研究所北京100044 [2]北京医科大学免疫学系,北京100083

出　　处：《中国实验血液学杂志》2000年第2期81-84,共4页Journal of Experimental Hematology

摘　　要：为探讨一个新的细胞凋亡相关基因———TFAR19所参与的凋亡途径 ,我们研究了几种不同方式诱导白血病细胞株Jurkat细胞凋亡过程中TFAR19表达的变化。采用去除血清 ,VP 16作用及Fas单抗活化受体等方法诱导Jurkat细胞凋亡后 ,以RT PCR方法检测mRNA水平TFAR19的表达 ,用流式细胞术及Western印迹检测蛋白水平TFAR19的表达。实验结果显示 ,Jurkat细胞在去除血清 12小时 ,VP 16作用 2小时 ,以及Fas单抗诱导细胞凋亡 2小时后 ,在mRNA及蛋白水平TFAR19的表达都呈增高趋势。结论提示 ,TFAR19参与了去除血清、DNA损伤及死亡受体激活所诱导的细胞凋亡过程 ,它在细胞凋亡过程的早期开始发挥作用 ,TFAR19是细胞凋亡途径中的“终末共同通路”的参与者。A new apoptosis related gene TFAR19 was recently cloned. A primary functional analysis indicates its role in the apoptotic process of TF 1 cells. To clarify TFAR19 was involved in which apoptotic pathway, the changes in TFAR19 expression were observed during the apoptotic processes of Jurkat cells induced with various methods: serum deprivation, VP 16 treatment and Fas McAb activation. Then TFAR19 expression in Jurkat cells was detected with flow cytometry and Western blot, and TFAR19 mRNA with RT PCR. Our results showed that expression of TFAR19 in Jurkat cells was increased at 12 hours after serum deprivation, 2 hours after VP 16 treatment and 2 hours after Fas McAb activation, respectively. These observations suggested that TFAR19 was involved in the apoptotic process of Jurkat cells induced with serum deprivation, DNA destruction and death receptor activation. It might take effect in the early apoptotic process. TFAR19 is a participant of the 'final common pathway' in the apoptotic pathway.

关 键 词：细胞凋亡 基因表达 TFAR19 白血病细胞株 

分 类 号：R73－36[医药卫生—肿瘤] R733.705[医药卫生—临床医学]