高压氧后处理对大鼠神经病理性痛及脊髓小胶质细胞活化的影响  

Effects of hyperbaric oxygen postconditioning of microglia on activation in rats model of neuropathic pain

作　　者：赵柏松[1] 丁远远[1] 王浩[1] 韩光[1] 孟凌新[1] 

机构地区：[1]中国医科大学附属盛京医院麻醉科,沈阳110004

出　　处：《国际麻醉学与复苏杂志》2013年第9期799-802,共4页International Journal of Anesthesiology and Resuscitation

基　　金：沈阳市科技计划项目（F10-205-1-41）;贝朗麻醉科学研究基金（BBF2012-012）

摘　　要：目的探讨高压氧（hyperbaricoxygen，HBO）后处理对大鼠神经病理性疼痛（neuropathicpain，NP）镇痛效果及脊髓小胶质细胞活化的影响。方法雄性SD大鼠24只，采用随机数字表法将大鼠分成4组（每组6只）：假手术组（S组）、坐骨神经慢性压迫组（C组）、HBO后处理2．0组（H2．0组）与HBO后处理2．5组（H2．5组）。采用坐骨神经慢性压迫损伤法建立NP模型，H2．0和H2．5组于术后1d开始给予相应压力的HBO后处理，1次，d连续7次，每天出HBO舱后1h测定各组大鼠机械缩足反射阈值（mechanicalwithdrawalthreshold，MWT）和热缩足反射潜伏期（thermalwithdrawallatency，TWL），术后7d测定MWT和TWL后处死，用免疫组化法测定大鼠脊髓小胶质细胞的活化情况。结果与s组比较，C组MWT降低及聊L缩短，脊髓内小胶质细胞数量与活化率上升为（156±9）个和44．9％（P〈0．05）；与C组比较，H2．0组和H2．5组MWT升高及TwL延长，H2．0组和小胶质细胞数量与活化率分别为（64±7）个和5．7％（P〈0．05），H2．5组小胶质细胞数量与活化率分别为（62~5）个和6．1％（P〈O．05）；H2．0组与H2．5组小胶质细胞数量与活化率差异无统计学意义（P〉0．05）。结论HBO后处理可减轻大鼠NP，其机制与抑制脊髓内小胶质细胞活化有关。Objective To investigate the antinociceptive effect of hyperbaric oxygen（HBO） on activation of microglia in rats with neuropathic pain （NP） produced by chronic constriction injury of the sciatic nerve. Methods Twenty four healthy male Sprague-DaMey rats were randomly divided into four groups （n=6）： Sham group （group S）, CCI group （group C）,2.0 ATA HBO post-conditioning group （group H2.0） and 2.5ATA HBO post-conditioning group （group H2.5）. Group CCI, group H2.0 and group H2.5 were received the surgery of the chronic constriction injury. One day later, surgery HBO of 2.0 ATA and 2.5 ATA was given respectively by hyperbaric oxygen cabin once a day for seven days. The mechanical withdrawal threshold （MWT） and thermal withdrawal latency （TWL） of rats in each group were measured respectively 1 h after the surgery every day. The rats were killed after 7 days when MWT and TWL had already been measured. The lumbar segment L4-5 of the spinal cord was removed. Immunohistochemical technique was used to detect the activation of microglias in the spinal cord. Results Compared with group S, MWT and TWL were significantly decreased and the activation of microglias in the spinal cord was significantly enhanced after surgery in group C（P〈0.05 ）. Compared with group C, MWT and TWL were significantly increased and the activation of microglias in the spinal cord was significantly inhibited after surgery in group H2.0 and H2.5 （P〈0.05）. There was no significant difference in the activation of spinal microglias between group H2.0 and H2.5. Conclusions , HBO post-conditioning may produce the antinociceptive effect by inhibiting activation of spinal microglias in rats model of neuropathic pain.

关 键 词：高压氧 神经病理性疼痛 小胶质细胞 镇痛 

分 类 号：R741[医药卫生—神经病学与精神病学]