DM/PM中核因子-κВ的表达及作用机制探讨  

The expression and role of NF-κВ in DM/PM

作　　者：杨玉花[1] 陈爱明[2] 王彩梅 代丽美 

机构地区：[1]苏州大学第三附属医院皮肤科(常州市第一人民医院皮肤科),江苏常州213003 [2]苏州大学附属第一医院皮肤科,江苏苏州215006 [3]江苏省宜兴市皮肤病医院,江苏宜兴214200 [4]山东省威海市妇女儿童医院皮肤科,山东威海264200

出　　处：《皮肤病与性病》2013年第4期189-192,共4页Dermatology and Venereology

摘　　要：目的通过研究NF-κВ在DM/PM患者骨骼肌的表达情况,以及DM患者血清体外对脐静脉内皮细胞(HUVEC)NF-κВ表达的影响,初步探讨NF-κВ在DM/PM中的相关作用的机制。方法采用免疫组织化法对25例DM/PM患者骨骼肌中的NF-κВ(P65)进行检测,取16例DM患者治疗前和6例治疗后的血清加入体外培养的HUVEC中,用细胞免疫组织化法检测HUVEC株NF-κВ(P65)的表达。结果 NF-κВ(P65)在DM/PM患者骨骼肌内的微血管内皮细胞和肌纤维中表达上调,与正常对照组相比差异有显著性(P<0.05),正常对照组无表达;DM患者治疗前血清体外作用HUVEC 6h后,(11.126±1.920)%细胞表达阳性,24h后细胞的阳性率上升到(17.048±1.140)%,差异有统计学意义(P<0.01);治疗后血清对脐静脉内皮细胞(HUVEC)的NF-KB(P65)阳性表达率分别为(6.797±0.609)%(6h)和(11.273±0.643)%(24h),二者均低于相应的治疗前表达率(P<0.01)。结论 NF-κВ在DM/PM肌肉微血管肌纤维的炎症性反应过程中发挥重要作用,其在DM患者血清中可能有激活NF-κВ的成分。Objective To investigate the role of NF-κB in the pathogenesis of DM / PM. Methods The expression of NF-κB（p65） in the skeletal muscle of 25 cases with DM/PM was detected with the immunohistochemistry staining methos. The HUVEC were treated with serum of the 16 pre-treatment and 6 post-treatment patients with DM in vitro, and the expression of NF-KB （p65）in HUVEC was also detected. Results Compared with the normal control, the expression of NF-κB（p65）in the skeletal muscle microvascular endothe- lial cells and muscle fibers of patients with DM/PM was increased significantly（ P〈0.05 ）. It was no expression in normal control. When the HUVEC were treated with the serum of pre-treated patients with DM for 6 hours, the positive rate was （11. 126±1. 920）% , after 24 hours was （ 17. 048± 1. 140） %. There was significant difference （ P〈0. 001 ）. The expression of NF-KB （ p65 ） in HUVEC treated with the serum of the post-treated patients with DM for 6 hours and 24 hours was （6. 797 ±0. 609 ） % and （ 11. 273±0. 643 ） % separately. Conclusion NF-κB play an important role in the muscle microvascular inflammatory response of the muscle fibers of patients with DM / PM. There may have the ingredients to activate NF-κB in the serum of patients with DM.

关 键 词：皮肌炎 多肌炎 核因子-κВ 脐静脉内皮细胞 

分 类 号：R363.21[医药卫生—病理学] R593.26[医药卫生—基础医学]