P53、P21与细胞周期调节蛋白在食管癌中的表达及意义  

The expression and meaning of esophagus cancer in P53,P21 and adjustable protein of cell cycle

作　　者：牛韵韵[1] 高巍[2] 张云汉[1] 晏梅[1] 高冬玲[1] 

机构地区：[1]河南医科大学第一附属医院病理科河南省肿瘤病理重点实验室,450052 [2]河南医科大学第一附属医院耳鼻喉科

出　　处：《中国民政医学杂志》2000年第6期339-341,350,共4页Medical Journal of Chinese Civil Administration

基　　金：河南省科委科技攻关资助!课题 1981170 2 45

摘　　要：目的 :探讨 P5 3、P2 1、cyclin D1 、CDk4基因及蛋白在食管癌发生中的意义及其相互间的作用。方法 :采用 L SAB免疫组化染色法 ,对 43例食管癌手术标本的 P5 3、P2 1、cyclin D1 、CDk4蛋白进行标记。结果 :在鳞癌组织中 ,四种抗体的阳性物质存在于细胞核中和或胞浆中 ,在正常鳞状上皮主要存在于核中 ,且从正常鳞状上皮不典型增生鳞癌组织 ,P5 3的阳性表达逐渐上升 ,P2 1的阳性表达却随之下降 ;cyclin D1 、CDk4的阳性率则随 P2 1的下降而呈上升趋势。统计学处理 ,正常上皮与癌组织之间四种抗体的阳性率有显著差异性 (P值均小于 0 .0 5 ) ;同时 ,随着癌分化程度下降 ,P5 3、P2 1的阳性率在 级与 级鳞癌之间差异有显著性 (P<0 .0 5 ) ,但未检出 cyclin D1 、CDk4与癌分化的关系。结论 :p5 3、p2 1、cyclin D1 、CDk4蛋白表达的变化是食管癌发生中的早期事件 ,其构成的调节通路障碍可能参与了食管鳞癌的发生。Objective:To explore the meaning and interaction P53,P21,cyclinD1,CDK4 and protein in occurrence of esophagus cancer.Methods:The P53,P21,cyclinD1,CDK4 in 43 specimens of esophagus cancer were signed by LSAB.Results:In the epidermoid cancer tissue,4 antibodies were in cell nucleus and in cytoplasm,however,in normal epidermoid,that were in cell nucleus and the positive expression of P53 increased gradually,at the same time,the positive expression of P21 decreased,but the positive rate of cyclinD1,CDK 4 showed increasing trend.There were significantly difference between normal epidermoid and 4 antibodies of cancer tissues ( P <0 05).the positive rate of P53,P21 were significant difference between epidermoid cancer Ⅰ and Ⅱ with decreasing of cancer differentiation,but there were no relativity between cyclinD4,CDK4 and cancer differentiation.Conclusions:The expression change of P53,P21,cyclinD1,CDK4 occurred in early of esophagus cancer.Their adjustable passage impediment may be contributed to the esophagus cancer. [

关 键 词：食管癌 细胞周期调控 免疫组织化学 

分 类 号：R735.103[医药卫生—肿瘤]