污水处理系统中染料脱色酵母菌的筛选及脱色性能  

Screening and Decolorizing Research for Decolorizing Yeasts from Wastewater Treatment Systems

作　　者：张昊[1] 李晶[1] 张帆[1] 蒿洪欣[1] 许园园[1] 刘冬[1] 韩新宽[1] 杨清香[1] 

机构地区：[1]河南师范大学生命科学学院,资源微生物与功能分子河南省高校重点实验室培育基地,河南新乡453007

出　　处：《河南师范大学学报（自然科学版）》2013年第4期113-116,共4页Journal of Henan Normal University(Natural Science Edition)

基　　金：国家自然科学基金(20677014);河南省教育厅自然科学研究项目(2011B180033),河南省教育厅自然科学研究项目(13B180084);河南省高校科技创新团队支持计划(13IRTSTHN009)

摘　　要：从某印染厂和制药厂的污水处理系统中筛选了21株脱色酵母菌,测定了各菌株对染料RB5,M-3BE、KNR的脱色能力以及锰依赖过氧化物酶(MnP)的产生情况.结果表明,培养72h时有18株酵母菌对RB5,M-3BE的脱色率超过90%,且检测到MnP活性.26SrDNA序列分析结果表明,脱色酵母菌主要属于Rhodotorula mucilaginosa,Dipodascus capitatus,Torulaspora delbrueckii,Candida parapsilosis,Candida zeylanoides和Debaryomyces hansenii.In this paper, 21 strains of dye-decolorization yeasts were isolated from different activated sludge samples in- cluding textile and pharmacy wastewater treatment systems. The abilities of decolorization and production of manganese-de- pendent peroxidase（MnP） by different strains were investigated with dyes （active black RBS, active red M-3BE and active blue KNR）, respectively. The results showed that 18 strains could decolorize RB5 and M-3BE more than 90G, and produce MnP within 72 h. According to the sequence analysis of 26S rDNA, the decolorization yeasts were identified as Rhodotorula muci- laginosa , Dipodascus capitatus , Torulaspora delbrueckii , Candida parapsilosis , Candida zeylanoides and Debaryomyces hansenii.

关 键 词：污水处理 酵母菌 染料脱色 锰依赖过氧化物酶 

分 类 号：Q939.9[生物学—微生物学]