HIV阳性女性患者P16^(INK4a)蛋白表达与HPV感染相关性分析  

The Relationship Between Expression of P16^(INK4a) Protein and HPV Infection in HIV-positive Women

作　　者：罗丽丽 陈淑慧[2] 熊艳[2] 蔡红兵[2] 

机构地区：[1]平顶山市新华区人民医院,河南平顶山467000 [2]武汉大学中南医院,湖北省肿瘤医学临床研究中心,肿瘤生物行为湖北省重点实验室,湖北武汉430071

出　　处：《肿瘤学杂志》2013年第8期600-604,共5页Journal of Chinese Oncology

摘　　要：[目的]了解HIV阳性感染者宫颈脱落细胞P16INK4a蛋白表达与HPV感染的相关性。[方法]采用通用引物PCR和反向点杂交技术建立的检测方法,对182例HIV阳性感染者和300例HIV阴性感染者检测23种HPV亚型,包括18种高危型和5种低危型。同时采用免疫组织化学技术(S-P染色法)检测宫颈脱落细胞中P16INK4a蛋白表达情况。[结果]HIV阳性女性HPV感染率(36.8%)及P16INK4a蛋白阳性表达率(18.7%)较HIV阴性者(19.3%、4.3%)高(P均为0.000)。HIV阳性患者中,HPV感染阳性者宫颈脱落细胞P16INK4a蛋白表达率为43.3%,HPV阴性者4.3%(P=0.000);HPV多型感染者宫颈脱落细胞P16INK4a蛋白表达率(60.9%)较HPV单型感染者高(34.1%)(P=0.042);高危型HPV感染者宫颈脱落细胞P16INK4a蛋白表达率(80.6%)较低危型HPV感染者高(0)(P=0.000)。HIV阴性患者中,HPV感染阳性者P16INK4a蛋白阳性表达率(5.2%)较HPV阴性者(1.3%)高(P=0.721);所检HPV阴性患者均为单一HPV感染;高危型HPV感染患者P16INK4a蛋白阳性表达率(40.0%)较低危型(1.9%)明显增高(P=0.018)。[结论]HIV阳性女性中HPV感染阳性、多型HPV感染及高危型HPV感染宫颈细胞P16INK4a蛋白的表达率更高。[Purpose]To investigate the relationship between the P16INK4a protein expression in cervical exfoliated cells of HIV-positive women and the HPV infection. [Methods] Twenty-three HPV subtypes,including 18 high-risk types and 5 low-risk types were detected by the methods of universal primer PCR and reverse dot blot technique in 182 HIV-positive women and 300 HIV-negative women. The P16INK4a protein of the cervical exfoliated cells was detected by im- munohistoehemistry （S-P staining）. [Results] In HIV-positive women, the infection rate of HPV （36.8%） and P16INK4a protein expression （18.7%） were higher than those in HIV-negative women （19.3% ,4.3%）（P=0.000）. In HIV-positive women,the P16INK4a protein expression of the cervical exfoliated cells with HPV infection was significantly higher （43.3%） than that in the women with negative HPV infection （4.3%）（P=0.000）. In the women with multiple HPV infection,the P16INK4a protein expression was higher （60.9%） than that in women with single HPV infection（34.1%）（P= 0.042）. In women with high-risk HPV infection,the P16INK4a protein expression was higher （80.6%） than that in women with low-risk HPV infection（0）（P=0.000）. In HIV-negative group, the expression of P16INK4a protein was higher than that in the HPV-infected women （5.2%） （P= 0.721 ）. All of the HIV-negative women were single infected by HPV. In the high-risk HPV in- fection （40.0%）,the P16INK4a protein expression was higher than that in the low-risk infected women （ 1.9% ） （P=0.018 ）. [ Conclusion ] In cervical exfoliated cells of HIV-positive women, the P16INK4a protein expression in the group with positive HPV infection,multiple HPV infection and high-risk HPV infection are significantly higher.

关 键 词：人类免疫缺陷病毒 人乳头瘤病毒 P16INK4A蛋白 

分 类 号：R737.33[医药卫生—肿瘤]