抑制PRR11基因表达对H1299细胞周期的影响  

RNAi-mediated PRR11 Depletion Influences Cell Cycle Regulation in Lung Cancer H1299 Cells

作　　者：张春冬[1,2] 王义涛[1,2] 张莹[1,2] 李轶[1,2] 朱慧芳[1,2] 蔡伟[1,2] 朱远远[1,2] 卜友泉[1,2] 

机构地区：[1]重庆医科大学生物化学与分子生物学教研室,重庆400016 [2]重庆医科大学分子医学与肿瘤研究中心,重庆400016

出　　处：《中国细胞生物学学报》2013年第8期1133-1140,共8页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(批准号:30801356;81171879);重庆市自然科学基金计划(批准号:cstc2013jcyjA10043)资助的课题~~

摘　　要：Proline-rich protein 11(PRR11)是一个与细胞周期调控和肿瘤发生发展均密切相关的新基因。前期研究表明,PRR11敲减后导致肿瘤细胞周期S期阻滞。该研究利用流式细胞检测和免疫荧光方法在人肺癌细胞系H1299中分析siRNA介导的PRR11表达沉默对细胞周期产生的影响,通过添加dNTPs分析核糖核苷酸还原酶表达下降在PRR11敲降导致的S期阻滞中的作用。Brdu标记结果表明,PRR11敲减阻滞细胞周期在S期,并且核糖核苷酸还原酶表达下降导致细胞周期阻滞。使用分裂期标记蛋白pHH3分析结果表明,PRR11下调表达同时也导致细胞在G2期产生阻滞,从而抑制细胞进入有丝分裂。研究结果表明,在细胞周期过程中抑制PRR11的表达会阻滞DNA合成和有丝分裂进行,推测PRR11异常表达可能导致细胞周期紊乱,从而促进肿瘤的发生发展。Proline-rich protein 1 I（PRRll） is a novel tumor associated gene, implicated in both cell cycle progression and lung cancer. Studies have showed that downregulation of PRR11 by siRNA resulted in cell cycle arrest at S phase in cancer cells. In the present study, we analyzed the molecular mechanism of downregulation of PRR11 caused cell cycle arrest in lung cancer cell with FCM analysis and IF staining. Brdu labelling showed that downregulation of PRR11 caused cell cycle S phase arrest, and the downregualtion of ribonucleotide reductase resulted in cell cycle arrest by adding dNTPs in vitro. Mitotic marker pHH3 labelling showed silencing PRR11 caused cell cycle arrest at G2 phase leading to inhibition in mitosis. Abnormal expression of PRR11 may result in dysregulation of cell cycle progression and promote tumorigenesis.

关 键 词：肺癌 细胞周期 PRR11 脱氧核糖核苷酸 

分 类 号：R730.2[医药卫生—肿瘤]