Oct4在乳腺癌组织中的表达及其对乳腺癌细胞侵袭性的影响  

Expression of Oct4 in Breast Cancer Tissue and Its Effect on Cell Invasion of Breast Cancer

作　　者：曹勇[1] 张蕴蕴[2] 谢佳[1] 曾晓华[3] 吴诚义[1] 

机构地区：[1]重庆医科大学附属第一医院内分泌乳腺外科,重庆400016 [2]重庆医科大学附属第一医院肿瘤内科,重庆400016 [3]重庆市肿瘤研究所,重庆400030

出　　处：《中国细胞生物学学报》2013年第8期1161-1165,共5页Chinese Journal of Cell Biology

基　　金：重庆市科委攻关计划(批准号:CSTC2011AC5189)资助的课题~~

摘　　要：探讨转录因子Oct4(octamer-binding transcription factor 4)在乳腺癌中的表达及其对乳腺癌细胞侵袭性的影响。该研究选取27例乳腺癌组织病人的肿瘤组织及癌旁组织,通过Western blot方法检测Oct4的蛋白水平。通过慢病毒介导的是shRNA干扰技术靶向沉默oct4基因的表达,Westernblot方法验证oct4基因沉默效果。细胞划痕实验和细胞侵袭实验检测oct4沉默对细胞迁移及侵袭能力的影响。Western blot检测oct4沉默对上皮–间质转化(EMT)的多个标志物的影响。结果显示,74%病人(20/27)中Oct4蛋白在乳腺癌组织的表达显著高于癌旁组织。慢病毒介导的shRNA能显著抑制细胞Oct4蛋白的水平。oct4基因沉默能抑制乳腺癌细胞的迁移及侵袭能力。oct4沉默后上皮细胞标志物E-cadhrin、alpha-catenin明显上调;而间质细胞标志物vimentin、N-cadherin则明显下降。以上结果表明,Oct4蛋白在乳腺癌组织的表达明显增高;oct4沉默可以显著抑制乳腺癌细胞的迁移及侵袭。This paper examined the expression level of Oct4 in breast cancer tissue and further investigate its effect on cell invasion of breast cancer. Western blot analysis was used to detect the protein level of Oct4 in 27 paired breast cancer tissue and adjacent non-tumoral breast tissue. Oct4 expression in breast cancer cells was knock- down by lentivirus-mediated shRNA interference technology. The effect of oct4 silencing on the cell invasion was determined by wound-healing assay and cell invasion assay. Four EMT markers （E-cadhrin, alpha-catenin, vimen-tin and N-cadherin） were analyzed by Western blot analysis. The results demonstrated that 74% （20/27） patients showed elevated Oct4 expression in breast cancer tissue compared with that in adjacent non-tumoral breast tissue. Lentivirus-mediated shRNA significantly suppressed Oct4 expression. Gene silencing of oct4 markedly inhibited invasion of breast cancer cells. Knockdown of oct4 in breast cancer cells induced the expression of epithelial mark- ers E-cadherin and et-catenin that was accompanied by a concomitant reduction of mesenchymal marker N-cadherin and vimentin. These finding suggested that Oct4 expression was upregualted in breast cancer tissue. Gene silencing of oct4 inhibited cell invasion of breast cancer through regulating EMT.

关 键 词：乳腺癌 肿瘤细胞系 肿瘤侵袭 OCT4 

分 类 号：R737.9[医药卫生—肿瘤]