不同途径与策略获取成熟肝(样)细胞的研究进展  

Research Progress in Generation of Mature Hepatocytes or Hepatocyte-like Cells by Different Ways and Strategies

作　　者：樊晋宇[1,2] 王妍[1,2] 李丽丽[1,2] 徐存拴 

机构地区：[1]河南省–科技部共建细胞分化调控国家重点实验室培育基地,新乡453007 [2]河南师范大学生命科学学院,新乡453007

出　　处：《中国细胞生物学学报》2013年第8期1196-1204,共9页Chinese Journal of Cell Biology

基　　金：河南省基础与前沿技术研究项目(批准号:092300410015;082300450560)资助的课题~~

摘　　要：肝细胞是肝脏生理功能的主要执行者,在肝脏疾病的细胞治疗与药物研究和开发中具有重要的理论与应用价值。直接分离的原代肝细胞很难在体外增殖培养,因此从其他途径获取大量成熟的肝细胞一直是备受关注的研究热点。该文简要综述了采用不同策略从肝干细胞、胚胎干细胞、其他成体祖/干细胞以及其他成熟体细胞诱导为成熟肝细胞或肝样细胞的研究成果与最新研究进展,并对该研究领域进行了小结与展望。Hepatocytes perform most of the liver＇s functions and are very important for the cellular ther- apy of liver disease as well as in drug research and development. It is difficult for primary cultured hepatocytes to expand and maintain for a long period in vitro, so how to get a large number of mature, fully differentiated hepato- cytes has always been regarded as one of the most important research problem. In this review, we are going to sum- marize the results and the latest research progress of derivation of mature hepatocytes or hepatocyte-like cells from liver stem ceils, embryonic stem cells, adult progenitor or stem cells as well as other adult cells via different strate- gies, with the expectation of directions for future research.

关 键 词：肝细胞 肝干细胞 胚胎干细胞 诱导多能干细胞 多能成体祖细胞 诱导分化 转分化 

分 类 号：R575[医药卫生—消化系统]