HPV16 E2对宫颈癌SiHa细胞上皮间质转化的影响  

Influence of HPV16 E2 on epithelial-mesenchymal transition of cervical cancer SiHa cells

作　　者：钱文艳[1,2] 张稼闻 董煜[1] 刘源[1] 石灿[1] 张箴波[1] 杨永彬[1] 丰有吉[1] 邬素芳[1] 

机构地区：[1]上海交通大学附属第一人民医院妇产科,上海201620 [2]昆山中医院,昆山215300

出　　处：《上海交通大学学报（医学版）》2013年第8期1074-1078,共5页Journal of Shanghai Jiao tong University:Medical Science

基　　金：上海市科委项目(09411962500);上海市科委浦江人才计划D类项目(12PJD002);国家自然基金青年项目(81201541);卫生部行业基金项目(201002013)~~

摘　　要：目的探讨HPV16 E2对宫颈癌SiHa细胞上皮间充质细胞转化的影响。方法采用脂质体转染p-CAG-MYC-HPV16 E2至宫颈癌SiHa细胞中,倒置相差显微镜下观察转染前后细胞形态的改变;RT-PCR及Western blotting检测转染前后Twist2、上皮细胞标志物E-cadherin及间质细胞标志物Vimentin表达的变化。结果成功转染HPV16 E2至宫颈癌SiHa细胞中。转染后,上皮细胞由椭圆形变成梭形,细胞间隙增大;Twist2和Vimentin表达上调,E-cadherin表达下调。结论 HPV16 E2能在体外诱导宫颈癌细胞发生上皮间充质细胞转化,促进早期癌变。Objective To investigate the influence of HPV16 E2 on epithelial-mesenchymal transition of cervical cancer SiHa ceils. Methods Lipofectamine was used to transfect p-CAG-MYC-HPV16 E2 gene into cervical cancer SiHa cells. The morphology of cells was observed before and after transfection with phase-contrast microscope, and the expression of Twist2, E-cadherin and Vimentin before and after transfection was determined by RT-PCR and Western blotting. Results HPV16 E2 was successfully transfected into SiHa cells. After transfection, the epithelial cells became spindle-shaped, with enlarged intercellular space, and the expression of Twist2 and Vimentin was up-regulated, while the expression of E-cadherin was down-regulated. Conclusion HPV16 E2 can induce epithelial-mesenchymal transition of cervical cancer cells, and promote cervical carcinogenesis.

关 键 词：HPV16 E2 上皮间质转化 转染 宫颈癌 

分 类 号：R737.33[医药卫生—肿瘤]