冻存H_(22)细胞在卡介苗抑瘤小鼠模型中的应用  

Application of H_(22) cells stored in frozen to mouse model of BCG tumor-suppressing test

作　　者：王鹏[1,2] 赵爱华[1] 寇丽杰[1] 乔来艳[1] 李贤珍[1] 岳婷婷[1] 王国治[1] 

机构地区：[1]中国食品药品检定研究院,北京100050 [2]中国人民解放军军事医学科学院,北京100850

出　　处：《中国生物制品学杂志》2013年第8期1100-1101,1106,共3页Chinese Journal of Biologicals

摘　　要：目的探讨冻存H22肝癌细胞应用于卡介苗抑瘤小鼠模型的可行性。方法制备冻存H22细胞,分别将冻存10和83 d的H22细胞复苏,计算细胞存活率;将高(7.5×106个/ml)、中(5.0×106个/ml)、低(2.5×106个/ml)剂量的新复苏的H22细胞与1 mg/ml治疗用卡介苗混合后经左侧背部皮下免疫BALB/c小鼠,0.1 ml/只,对照组只接种相应浓度的H22细胞,接种后30 d,称量小鼠皮下瘤体重量,并计算试验组的抑瘤率,试验重复2次。结果冻存10和83 d的H22细胞复苏后的存活率分别为85.45%和82.25%。所有对照组小鼠接种H22细胞后均有肿瘤形成,成瘤率均为100%;同一次试验中,各试验组瘤体重量均明显小于同剂量对照组(P<0.05);同一次试验中,各试验组间瘤体重量差异无统计学意义(P>0.05);不同次试验中,同剂量对照组间瘤体重量差异均有统计学意义(P<0.05);各试验组的抑瘤率均大于60%。结论冻存H22细胞可满足抑瘤试验需要,但试验稳定性仍需改进。Objective To investigate the feasibility of application of liver cancer H22 cells stored in frozen to mouse model of BCG tumor-suppressing test.Methods H22 cells stored in frozen were prepared,of which those 10 and 83 d after storage were resuscitated and calculated for survival rate.The newly resuscitated H22 cells at high（7.5 × 106 cells / ml）,moderate（5.0 × 106 cells / ml）and low（2.5 × 106 cells / ml）were mixed with 1 mg / ml therapeutic BCG respectively,with which BALB / c mice were inoculated subcutaneously,0.1 ml for each,using those with H22 cells at the corresponding dosages as control.The tumors of mice 30 d after inoculation were weighed,based on which the tumor-suppressing rate in test groups were calculated.The test was repeated.Results The survival rates of resuscitated H22 cells 10 and 83 d after storage in frozen were 85.45% and 82.25% respectively.The tumor formation rates in control groups were 100%.In the same test,the tumor weights were significantly lower in test groups than in the corresponding control groups（P 0.05）,while showed no significant difference in various test groups（P 0.05）.However,in different tests,the tumor weights of mice inoculated with H22 cells at the same dosages were significantly differen（t P 0.05）.All the tumor-suppressing rates in various test groups were more than 60%.Conclusion The H22 cells stored in frozen met the requirements for tumor-suppressing test,while the stability of test needed to be improved.

关 键 词：冻存细胞 H22肝癌细胞 卡介苗 抑瘤试验 BALB C小鼠 

分 类 号：R735.7[医药卫生—肿瘤]