团头鲂幼鱼吡哆醇适宜需求量的研究  

OPTIMAL DIETARY PYRIDOXINE REQUIREMENT OF JUVENILE BLUNT SNOUT BREAM, MEGALOBRAMA AMBLYCEPHALA

作　　者：王莹[1,2] 李向飞[1] 张微微[1] 徐维娜[1] 刘文斌[1] 

机构地区：[1]南京农业大学动物科技学院,江苏省水产动物营养重点实验室,南京210095 [2]南京农业大学无锡渔业学院,无锡214081

出　　处：《水生生物学报》2013年第4期632-639,共8页Acta Hydrobiologica Sinica

基　　金：农业公益性行业科研专项-水产养殖动物营养需求与高效配合饲料开发(项目编号:201003020);现代农业产业技术体系建设专项-国家大宗淡水鱼类产业技术体系资金(项目编号:CARS-46-20)资助

摘　　要：试验采用单因素试验设计,以饲料中吡哆醇浓度为影响因素,研究了团头鲂幼鱼的适宜吡哆醇需求量。试验共配置了7组等氮等能的半纯化饲料,其吡哆醇的实际含量分别为0、1.04、1.99、4.07、5.91、7.96和9.22 mg/kg。选用840尾[均重:(6.81±0.17)g]团头鲂幼鱼,随机分为7组,每组4重复,每重复30尾鱼,日投饵3次,养殖期为8周。结果表明,当饲料中吡哆醇含量由0升高至5.91 mg/kg时,团头鲂的增重率、特定生长率、饲料利用率、成活率、蛋白效率比和氮保留率均得到显著改善(P<0.05);当吡哆醇含量进一步升高至9.22 mg/kg时,蛋白效率比和氮保留率均显著下降(P<0.05),而其他指标则无显著变化(P>0.05)。饲料中的吡哆醇含量显著影响团头鲂的肝体比(P<0.05)且以5.91 mg/kg组为最低,但对肥满度和胴体率均无显著影响(P>0.05)。当饲料吡哆醇含量由0升高至5.91 mg/kg时,肝脏谷草转氨酶和谷丙转氨酶活性以及吡哆醇含量均显著升高(P<0.05);当吡哆醇含量进一步升高至9.22 mg/kg时,三者均无显著变化(P>0.05)。以肝脏中的谷丙和谷草转氨酶活性以及吡哆醇含量为评价指标,拟合折线模型得到团头鲂幼鱼的适宜吡哆醇的需求量为4.17—5.02 mg/kg。This study was conducted to determine the optimal dietary pyridoxine requirement of juvenile blunt snout bream Megalobrama amblycephala.Eight hundred and forty fishes [average initial weight：（6.81±0.17） g] were fed with seven isonitrogenous and isoenergetic diets containing different pyridoxine levels（0,1.04,1.99,4.07,5.91,7.96 and 9.22 mg/kg） 3 times daily for 8 weeks,respectively.Each diet was tested in four replicates,and each replicate had 30 fishes.Weight gain（WG）,specific growth rate（SGR）,feed conversion ratio（FCR）,survival rate,protein efficiency ratio（PER） and nitrogen retention efficiency（NRE） were all increased significantly（P〈0.05） as dietary pyridoxine levels increased from 0 to 5.91 mg/kg.PER and NRE were all decreased significantly（P〈0.05） with further increase of pyridoxine levels,but WG,SGR and FCR showed no significant difference（P〈0.05）.No significant difference（P〈0.05） was observed both in condition factor and dressout percentage among all the treatments,while hepatosomatic index was significantly（P〈0.05） affected by dietary pyridoxine levels.The lowest value was observed in fish fed with 5.91 mg/kg pyridoxine.Liver glutamic-pyruvic transaminase（GPT） and glutamic-oxalpacetate transaminase（GOT） activities and pyridoxine contents all increased significantly（P〈0.05） as dietary pyridoxine levels increased from 0 to 5.91 mg/kg.However,they all showed no significant difference（P〈0.05） with further increase of pyridoxine levels.On the basis of the broken-line analysis of hepatic GOT and GPT activities and liver pyridoxine contents against dietary pyridoxine levels respectively,the optimal dietary pyridoxine requirement of juvenile blunt snout bream was estimated to be 4.17—5.02 mg/kg.

关 键 词：团头鲂 吡哆醇 生长性能 转氨酶活性 

分 类 号：S963.73[农业科学—水产养殖]