鞘脂激活蛋白C通过上调雄激素受体的表达与活性促进LNCaP细胞的增殖  

Saposin C promotes proliferation of LNCaP cells via up-regulating the expression and activity of androgen receptor

作　　者：任凯[1] 丁岩[2] 成凡[1] 付慧[1] 张雅利[2] 

机构地区：[1]西安医学院生物化学教研室,陕西西安710021 [2]西安交通大学生命科学与技术学院,陕西西安710049

出　　处：《西安交通大学学报（医学版）》2013年第5期619-622,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助项目(No.31101266);陕西省教育厅科研计划项目资助项目(No.12JK0711)~~

摘　　要：目的构建含有鞘脂激活蛋白C(Saposin C)的真核表达载体,探讨Saposin C在前列腺癌雄激素依赖性前列腺细胞(LNCaP)中对雄激素受体(AR)及细胞增殖的影响。方法通过cDNA法构建含有TAT和Saposin C的真核表达载体,并将其转染至前列腺癌LNCaP细胞。通过MTS、RT-PCR、Western blot等实验,分别检测TAT-Saposin C与Saposin C的AR基因表达与其转录激活功能,以及对前列腺癌细胞增殖的影响。结果在不依赖雄激素的情况下,Saposin C能够上调AR与PSA基因mRNA和蛋白质水平的表达,增加AR的转录激活活性,刺激LNCaP细胞的增殖。结论 Saposin C通过雄激素非依赖性上调AR的表达与转录激活活性,促进LNCaP细胞的增殖。Objective To construct eukaryotic expression vectors containing Saposin C so as to investigate the effects of Saposin C on androgen receptor （AR） expression and proliferation of LNCaP cells. Methods Recombination vectors pcDNA-TAT-Saposin C and pcDNA-Saposin C were constructed using cDNA methods. After the vectors were transfected into LNCaP cells, the proliferation of LNCaP cells was assessed by MTS assay. Expression of AR in TAT-Saposin C and Saposin C and transcriptional activation were examined by Western blot and RT-PCR. Results In the absence of androgen, Saposin C up-regulated AR and PSA expressions at the protein and mRNA levels, increased transcriptional activation of AR, and stimulated the proliferation of LNCaP cells. Conclusion Saposin C promotes the proliferation of LNCaP cells by up-regulating AR expression and transcriptional activation in an androgen-independent manner.

关 键 词：鞘脂激活蛋白C 雄激素受体 前列腺特异性抗原 前列腺癌 细胞增殖 雄激素依赖性前列腺细胞 

分 类 号：R737.25[医药卫生—肿瘤]