miR-145对口腔癌细胞TCA8113生物学特性的影响  

Effects of miR-145 on the biological characteristics of human oral carcinoma cell line TCA8113

作　　者：邵渊[1] 张少强[1] 权芳[1] 张鹏飞[1] 吴胜利[2] 

机构地区：[1]西安交通大学医学院第一附属医院耳鼻喉科,陕西西安710061 [2]西安交通大学医学院第一附属医院肝胆外科,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2013年第5期652-655,663,共5页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助项目(No.30901946/H2902)~~

摘　　要：目的探讨miR-145对口腔癌细胞增殖、迁移、侵袭生物学特性的影响。方法运用脂质体介导的转染方法将miR-145转染入口腔癌细胞TCA8113,通过细胞增殖实验检测转染后口腔癌细胞TCA8113增殖生物学特性的改变,通过细胞迁移实验、细胞侵袭实验,检测口腔癌细胞TCA8113细胞迁移、侵袭生物学特性的变化。结果 miR-145转染组细胞增殖活性明显低于对照组(P<0.05),细胞迁移、侵袭活性明显低于对照组(P<0.05)。结论 miR-145的表达参与口腔细胞癌的发生及发展过程,miR-145的高表达可对口腔癌细胞TCA8113发挥负性调控作用。Objective To investigate the effects of miR-145 on the biological characteristics including proliferation, migration and invasion of oral carcinoma cell TCA8113. Methods The lipofectamine transfection method was used to improve the miR-145 expression in oral carcinoma TCA8113. The biological characteristics changes of oral carcinoma cell TCA8113 in vitro were assayed by proliferation test, migration test, and invasion test. Results After miR-145 transfection, the proliferation, migration and invasion characteristics of oral carcinoma TCA8113 were markedly decreased compared with those in control group （P〈0.05）. Conclusion The expression of miR-145 is associated with the genesis and development of oral carcinoma. Overexpression of miR-145 can negatively regulate oral carcinoma TCA8113.

关 键 词：口腔癌细胞 MIR-145 增殖 迁移 侵袭 

分 类 号：R739.8[医药卫生—肿瘤]