基因组改组选育产酯化酶地衣芽孢杆菌  

Breeding of Esterifying Enzyme-producing Bacillus licheniformis by Genome Shuffling

作　　者：薛正莲[1] 刘阳[1] 王洲[1] 马琦亚[1] 赵世光[1] 苏燕南[1] 

机构地区：[1]安徽工程大学生物与化学工程学院微生物发酵安徽省工程技术研究中心,芜湖241000

出　　处：《中国生物工程杂志》2013年第8期43-48,共6页China Biotechnology

摘　　要：从高温大曲中筛选到一株产酯化酶的地衣芽胞杆菌G2。采用低温等离子体对G2进行诱变,以筛选酶活提高最多的3株菌株作为亲本。将亲本原生质体灭活后对其进行基因组改组。将融合后的菌体进行筛选纯化之后,对融合子进行发酵测定其酶活并挑选酶活较高菌株进行传代测定其酶活稳定性。结果表明6#融合子酶活最高且稳定为10.7U/ml,相对于初始菌株的5.3U/ml提高了102%。对其发酵液进行气相色谱分析表明,6#融合子代谢产一定量的酸类、酯类及醇类等物质,其中乙偶姻含量达2565mg/L。A Bacillus licheniformis strain named G2 with esterifying enzyme activity screened from high temperature Daqu was mutagenized by using low temperature plasma and three strains with high esterifying enzyme activity was screened as parents . The parent protoplast was inactivated for genome shuffling. After the fused strains were purified by selection, the enzyme activity of fusants was determinated by fermentation, the strains of higher enzyme activity were screened to passage and their stability of enzyme activity were detected. The highest and stable enzyme activity of 6# fusant can reach 10. 7U/ml , which has been improved by 102%compared with the 5.3U/ml of original strain G2. The fermentation broth of the 6# fusant was analyzed by GC , the results show that the strain can produce a certain amount of acids , alcohols and esters, and the acetoin of thefermentation broth can reach 2565mg/L.

关 键 词：酯化酶 基因组改组 地衣芽孢杆菌 等离子诱变 

分 类 号：Q819[生物学—生物工程]