共培养下前交叉韧带成纤维细胞中LOXs与MMPs的基因表达情况  

Gene Expressions of LOXs and MMPs of the ACL Fibroblasts Cells Co-cultured with Synovial Cells

作　　者：王春莉[1] 梅虎[1] 谢静[1] 蒋稼欢[1] 陈荣富[3] 尹琳[1] 符纯锋[3] 陈诚[3] 宋国立[1,2] 

机构地区：[1]重庆大学生物工程学院,重庆400044 [2]加州大学圣迭亚哥分校骨科系及生物工程系,美国加利福尼亚州92093-0412 [3]重庆医科大学附属第一医院骨科,重庆400016

出　　处：《生物医学工程学杂志》2013年第4期777-781,共5页Journal of Biomedical Engineering

摘　　要：前交叉韧带(ACL)损伤修复的研究进展表明,膝关节内覆在ACL上的滑膜组织对ACL损伤修复起到非常重要的作用。为探讨滑膜细胞对ACL成纤维细胞中影响组织修复的关键酶即赖氨酰氧化酶(LOXs)与基质金属蛋白酶(MMPs)表达及活性的影响,本文通过建立体外ACL成纤维细胞与滑膜细胞共培养体系,采用RT-PCR与明胶酶谱(zemography)技术定量分析比较单培养与共培养ACL成纤维细胞中LOXs与MMP-1、2、3基因的表达情况及MMP-2蛋白酶的活性。结果显示:(1)共培养促进ACL成纤维细胞中的LOXs与MMP-2的基因表达,但降低MMP-1、MMP-3的基因表达。(2)与单培养组相比,在不同时间点,共培养组都促进ACL成纤维细胞中MMP-2蛋白酶的表达及其活化程度。以上结果表明:ACL细胞和滑膜细胞之间存在密切的交流影响了LOXs与MMP-1、2、3的基因表达,为后续探索ACL损伤修复的机制及治疗方法提供了理论依据。The progress of research on the the anterior cruciate ligament（ACL） wound healing demonstrates that the synovial tissue in the knee joint plays a very important role in the healing process of injured ACL.Therefore,the molecular response mechanisms of lysyl oxidase（LOX） and matrix metalloproteina（MMP） in normal/injured ACL fibroblast cells could be considered to perform the major analysis function of injured ACL healing mechanism.The mRNA expressions of LOXs and MMPs and the activity expressions of MMP-2 in ACL fibroblasts co-cultured with synovial cells were analyzed by quantitative real-time PCR and zymography.The results showed that co-culture could regulate the mRNA expressions of LOXs and MMPs in the ACL fibroblasts cells.These results suggest that the differential expressions of LOXs and MMP-1,2,3 in co-cultured ACL indicate that interaction crosstalk do exist between ACL cells and synovial cells and provide a theoretical basis for subsequent exploration of the mechanisms and treatment of ACL injury and repair.

关 键 词：前交叉韧带 滑膜 共培养 赖氨酰氧化酶 基质金属蛋白酶 

分 类 号：R363[医药卫生—病理学]