血红素加氧酶-1在高糖诱导的间充质干细胞损伤中的作用研究  

The Effect of Heme Oxygenase-1 on BMSCs Damaged by High-concentration Glucose

作　　者：侯春丽[1] 沈雷 吴杨霄[1] 杨明璨[1] 曾文[1] 李立[1] 陈文[1] 朱楚洪[1] 

机构地区：[1]第三军医大学人体解剖学教研室,国家教育部生物力学与组织工程重点实验室生物力学室,重庆400038 [2]齐齐哈尔医科大学人体解剖学教研室,齐齐哈尔161006

出　　处：《生物医学工程学杂志》2013年第4期798-802,共5页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(31100707);重庆市自然科学基金资助项目(CSTC;2010BB5176)

摘　　要：本研究旨在探究血红素加氧酶-1(HO-1)高表达能否抑制高糖诱导的骨髓间充质干细胞(BMSCs)的损伤。实验采用高糖刺激BMSCs,通过激动剂和抑制剂分别上调和抑制BMSCs内HO-1的表达。通过MTT和ELISA法分别检测BMSCs的增殖及分泌生长因子的能力。通过划痕实验以及Transwell实验检测BMSCs条件培养基对人脐静脉血管内皮细胞(HUVECs)增殖及迁移能力的影响。研究发现,HO-1高表达不但可以促进BMSCs自身增殖,而且可以促进BMSCs分泌血管内皮生长因子(VEGF),进而加速HUVECs的增殖和迁移。结果表明,HO-1高表达的BMSCs不但可以抑制高糖损伤,而且可通过旁分泌促进血管内皮细胞的增殖和迁移,可能对糖尿病血管并发症的治疗具有重要作用。This investigation was aimed to explore whether over-expression of 27heme oxygenase-1（HO-1） could protect bone marrow mesenchymal stem cells（BMSCs）against injury induced by high-concentration glucose.We cultured BMSCs in high-concentration glucose medium,and up-regulated or inhibited HO-1 expression in BMSCs through its agonist or inhibitor.We detected the ability of BMSCs proliferation and secretion respectively by MTT and enzyme-linked immunosorbnent assay（ELISA）.Then we detected the effect of BMSCs conditions medium on proliferation and migration of human umbilical vein endothelial cells（HUVECs） through scratch experiments and transwell assay.It was found that HO-1 over-expression could not only promote BMSCs proliferation,but also promote secretion of vascular endothelial growth factor（VEGF）,and could further accelerate the proliferation and migration of HUVECs.It could be well concluded that HO-1-over-expressing BMSCs can not only inhibit damage induced by high-concentration glucose,but can promote the proliferation and migration of vascular endothelial cells through paracrine as well.The result indicated that HO-1-over-expressing BMSCs played an important role in the treatment of diabetic vascular complication.

关 键 词：高糖 血红素加氧酶-1 骨髓间充质干细胞 脐静脉内皮细胞 

分 类 号：R318.06[医药卫生—生物医学工程] R329.28[医药卫生—基础医学]