三种方法提取当归挥发性成分的气相色谱-质谱联用分析  

Analysis of Three Methods for Extracting Volatile Constituent from DangGui by Combination of Gas Chromatography and Mass Spectrometry

作　　者：李冬华 马潇 宋平顺 赵建邦 丁永辉 

机构地区：[1]甘肃省食品药品检验所,甘肃兰州730000 [2]甘肃省中药品质与安全评价工程技术研究中心 [3]甘肃省食品药品监督管理局

出　　处：《西部中医药》2013年第8期15-18,共4页Western Journal of Traditional Chinese Medicine

基　　金：甘肃省科技重大专项计划(当归等八种道地药材质量标准制订)(编号1002FKDA048)

摘　　要：目的:对甘肃产当归的挥发性成分进行分析。方法:分别采用常压水蒸气蒸馏法、乙醚超声提取法、超临界萃取法提取当归挥发油,利用气相色谱-质谱联用技术(GC-MS)对提取物中的挥发性成分进行分离分析,并计算各成分的相对百分含量。结果:从常压水蒸气蒸馏法提取的当归挥发油中共鉴定出29种成分,乙醚超声提取法提取的当归挥发油中共鉴定出27种成分,超临界萃取法提取的当归挥发油中共鉴定出25种成分。3种方法提取的挥发油无论在成分还是含量上均存在一定差异,但也存在相同点,主要成分均为藁本内酯,在常压水蒸气蒸馏法中占55.89%,在乙醚超声提取法法中占78.27%,在超临界萃取法中占78.19%。结论:用常压水蒸气蒸馏法提取的当归挥发油中藁本内酯的含量较低,而用乙醚超声提取法和超临界萃取法提取的当归挥发油中藁本内酯的含量较高,二者相当。Objective： To analyze volatile constituents from Gansu DangGui（angelica sinesis）.Method： Volatile constituents of DangGui were extracted with the method of normal pressure steam distillation（NPSD）,aether ultrasonic extraction and supercritical CO2fluid extraction（SFE-CO2）,volatile ingredients were separated and analyzed by combination of gas chromatography and mass spectrometry,their relative percentage were calculated.Result： Altogether 29 kinds of ingredients were identified from volatile oil extracted from NPSD,27 kinds of ingredients from volatile oil extracted from aether ultrasonic extraction and 25 kinds from volatile oil from SFE-CO2.There were certain differences among the volatile oil extracted from three methods in the ingredients and contents,and also some similarities,the main ingredient was ligustilide,which reached 55.89% with the method of NPSD,78.27% with the method of aether ultrasonic extraction and 78.19% with SFE-CO2.Conclusion： Contents of ligustilide with the method of NPSD are less than obtained ligstilide by the methods of SFE-CO2and aether ultrasonic extraction.

关 键 词：当归 挥发油 水蒸气蒸馏法 超临界萃取法 气相色谱-质谱联用 

分 类 号：R284[医药卫生—中药学]